# Third national assessment

- Executive Summary
- 1.0 Introduction
- 2.0 Methods
- 3.0 Presence or Absence of Effects
- 4.0 Effluent Quality
- 5.0 Biological Monitoring Studies Investigating Observed Effects
- 6.0 Key Findings
- 7.0 Glossary
- 8.0 References
- Appendix A: Metal Mines Subject to the Metal Mining Effluent Regulations in 2013
- Appendix B: Effect Indicators, Critical Effects Sizes and Studies Conducted
- Appendix C: Mine-by-Mine Results of Studies Assessing Potential Effects
- Appendix D: Fish Tissue Mean Total Mercury Concentrations per Mine
- Appendix E: Trends in Sublethal Toxicity
- Appendix F: Trends in Sublethal Toxicity for Ore Types
- Appendix G: Annual Mean Concentrations of Effluent Characterization Data
- Appendix H: Mine-by-Mine Summary of Investigation Studies

## Appendix F: Trends in Sublethal Toxicity (SLT) for Ore Types

**Figure F1**. Geometric mean IC_{25} (percent effluent on a volume basis) each year for each SLT test at precious metal mines

**Description**

Figure F1 is a line chart illustrating the annual geometric mean IC_{25} for each of five different sublethal toxicity tests conducted between 2003 and 2012 for precious metal mines. The annual geometric means for fish larval growth inhibition on *Pimephales promelas* are 75, 70, 93, 99, 100, 98, 96, 92, 97 and 95 and range in number of tests was 48-67. The annual geometric means for alga growth inhibition on *Pseudokirchneriella subcapitata* are 51, 57, 61, 66, 62, 68, 85, 79, 84 and 62 and range in number of tests was 51-72. The annual geometric means for plant growth inhibition on *Lemna minor* dry weight are 45, 46, 44, 66, 61, 59, 57, 47, 50 and 63 and range in number of tests was 50-68. The annual geometric means for plant growth inhibition on *Lemna minor* frond number are 25, 38, 30, 45, 35, 38, 35, 30, 38 and 40 and range in number of tests was 48-68. The annual geometric means for invertebrate reproduction inhibition on *Ceriodaphnia dubia* are 34, 35, 37, 45, 48, 48, 34, 53, 32 and 28 and range in number of tests was 52-69.

Note: Range in number of tests conducted per year in parentheses.

**Figure F2**. Geometric mean IC_{25} (percent effluent on a volume basis) each year for each SLT test at base metal mines

**Description**

Figure F2 is a line chart illustrating the annual geometric mean IC_{25} for each of five different sublethal toxicity tests conducted between 2003 and 2012 for base metal mines. The annual geometric means for fish larval growth inhibition on *Pimephales promelas* are 81, 82, 89, 81, 74, 82, 77, 86, 82 and 78 and range in number of tests was 39-59. The annual geometric means for alga growth inhibition on *Pseudokirchneriella subcapitata* are 50, 35, 41, 33, 60, 51, 51, 74, 81 and 60 and range in number of tests was 46-65. The annual geometric means for plant growth inhibition on *Lemna minor* dry weight are 46, 30, 44, 42, 36, 44, 32, 40, 28 and 43 and range in number of tests was 41-58. The annual geometric means for plant growth inhibition on *Lemna minor* frond number are 18, 18, 21, 19, 19, 26, 20, 17, 15 and 33 and range in number of tests was 39-61. The annual geometric means for invertebrate reproduction inhibition on *Ceriodaphnia dubia* are 23, 17, 23, 28, 26, 15, 15, 14, 15 and 24 and range in number of tests was 45-66.

Note: Range in number of tests conducted each year in parentheses.

**Figure F3**. Geometric mean IC_{25} (percent effluent on a volume basis) each year for each SLT test at uranium mines

**Description**

Figure F3 is a line chart illustrating the annual geometric mean IC_{25} for each of five different sublethal toxicity tests conducted between 2003 and 2012 for uranium mines. The annual geometric means for fish larval growth inhibition on *Pimephales promelas* are 96, 97, 100, 100, 100, 100,100, 100, 95 and 100 and range in number of tests was 7-15. The annual geometric means for alga growth inhibition on *Pseudokirchneriella subcapitata* are 82, 80, 80, 69, 54, 73, 85, 91, 75 and 91 and range in number of tests was 7-15. The annual geometric means for plant growth inhibition on *Lemna minor* dry weight are 23, 29, 24, 24, 26, 65, 78, 46, 61 and 97 and range in number of tests was 4-15. The annual geometric means for plant growth inhibition on *Lemna minor* frond number are 11, 16, 18, 8, 7, 30, 9, 7, 11 and 31 and range in number of tests was 7-15. The annual geometric means for invertebrate reproduction inhibition on *Ceriodaphnia dubia* are 22, 40, 42, 66, 12, 26, 37, 26, 33 and 34 and range in number of tests was 7-15.

Note: Range in number of tests conducted each year in parenthesis.

**Figure F4**. Geometric mean IC_{25} (percent effluent on a volume basis) each year for each SLT test at mines with “other” ore types

**Description**

Figure F4 is a line chart illustrating the annual geometric mean IC_{25} for each of five different sublethal toxicity tests conducted between 2003 and 2012 for other ore mines. The annual geometric means for fish larval growth inhibition on *Pimephales promelas* are 77, 74, 100, 89, 100, 88, 85, 100, 100 and 100 and range in number of tests was 5-11. The annual geometric means for alga growth inhibition on *Pseudokirchneriella subcapitata* are 82, 73, 61, 58, 30, 91, 62, 82, 62 and 78 and range in number of tests was 5-10. The annual geometric means for plant growth inhibition on *Lemna minor* dry weight are 33, 7, 18, 23, 14, 21, 22, 37, 19 and 8 and range in number of tests was 5-11. The annual geometric means for plant growth inhibition on *Lemna minor* frond number are 8, 9, 7, 12, 9, 24, 7, 10, 8 and 6 and range in number of tests was 5-10. The annual geometric means for invertebrate reproduction inhibition on *Ceriodaphnia dubia* are 27, 71, 37, 34, 15, 14, 18, 3, 9 and 12 and range in number of tests was 5-10.

Note: Range in number of tests conducted each year in parentheses.

**Figure F5**. Geometric mean IC_{25} (percent effluent on a volume basis) each year for each SLT test at iron ore mines

**Description**

Figure F5 is a line chart illustrating the annual geometric mean IC_{25} for each of five different sublethal toxicity tests conducted between 2003 and 2012 for iron ore mines. The annual geometric means for fish larval growth inhibition on *Pimephales promelas* are 46, 36, 21, 44, 21, 72, 91, 84, 100 and 72 and range in number of tests was 4-13. The annual geometric means for alga growth inhibition on *Pseudokirchneriella subcapitata* are 79, 62, 93, 7, 98, 84, 91, 91, 82 and 79 and range in number of tests was 4-14. The annual geometric means for plant growth inhibition on *Lemna minor* dry weight are 98, 25, 61, 98, 98, 97, 96, 80, 80 and 97 and range in number of tests was 2-10. The annual geometric means for plant growth inhibition on *Lemna minor* frond number are 26, 16, 11, 1, 8, 88, 92, 76, 82 and 83 and range in number of tests was 2-11. The annual geometric means for invertebrate reproduction inhibition on *Ceriodaphnia dubia* are 4, 2, 5, 15, 7, 30, 82, 84, 37 and 100 and range in number of tests was 4-12.

Note: Range in number of tests conducted each year in parentheses.

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