# Third national assessment

- Executive Summary
- 1.0 Introduction
- 2.0 Methods
- 3.0 Presence or Absence of Effects
- 4.0 Effluent Quality
- 5.0 Biological Monitoring Studies Investigating Observed Effects
- 6.0 Key Findings
- 7.0 Glossary
- 8.0 References
- Appendix A: Metal Mines Subject to the Metal Mining Effluent Regulations in 2013
- Appendix B: Effect Indicators, Critical Effects Sizes and Studies Conducted
- Appendix C: Mine-by-Mine Results of Studies Assessing Potential Effects
- Appendix D: Fish Tissue Mean Total Mercury Concentrations per Mine
- Appendix E: Trends in Sublethal Toxicity
- Appendix F: Trends in Sublethal Toxicity for Ore Types
- Appendix G: Annual Mean Concentrations of Effluent Characterization Data
- Appendix H: Mine-by-Mine Summary of Investigation Studies

## Appendix E: Trends in Sublethal Toxicity

**Figure E1**. Geometric mean IC_{25} (percent effluent on a volume basis) and proportion of tests in each sublethal toxicity category per year for the invertebrate reproduction inhibition test using *Ceriodaphnia dubia*

**Description**

Figure E1 is a line chart stacked above a proportional bar chart. The line chart illustrates the geometric mean IC_{25} per year from 2003 to 2012 for the invertebrate reproduction inhibition test using *Ceriodaphnia dubia*. The geometric mean IC_{25} per year was 25, 24, 28, 37, 30, 26, 26, 28, 23 and 29. The bar chart illustrates the proportion of tests in three sublethal toxicity categories that include higher toxicity IC_{25} ≤20%, lower toxicity IC_{25}>20% and <100% and no toxicity IC_{25}≥100%. The number of tests in the higher category per year was 49, 63, 50, 35, 45, 49, 47, 41, 53 and 47. The number of tests in the lower category per year was 44, 53, 52, 47, 26, 33, 35, 35, 39 and 40. The number of tests in the no toxicity category per year was 39, 39, 44, 47, 50, 52, 53, 61, 49 and 61. The total number of tests conducted per year was 132, 155, 146, 129, 121, 134, 135, 137, 141 and 148.

Note: The number of tests conducted each year ranged from 121 to 155.

**Figure E2**. Geometric mean IC_{25} (percent effluent on a volume basis) and proportion of tests in each sublethal toxicity category each year for the plant growth inhibition test using *Lemna minor* frond number

**Description**

Figure E2 is a line chart stacked above a proportional bar chart. The line chart illustrates the geometric mean IC_{25} per year from 2003 to 2012 for the plant growth inhibition test using, *Lemna minor* frond number. The geometric mean IC_{25} per year was 19, 22, 22, 25, 21, 32, 25, 22, 25, and 36. The bar chart illustrates the proportion of tests in three sublethal toxicity categories that include higher toxicity IC_{25} ≤20%, lower toxicity IC_{25}>20% and <100% and no toxicity IC_{25}≥100%. The number of tests in the higher category per year was 65, 56, 54, 47, 47, 45, 42, 49, 49 and 38. The number of tests in the lower category per year was 37, 42, 25, 19, 28, 39, 47, 48, 47 and 59. The number of tests in the no toxicity category per year was 27, 37, 44, 50, 42, 51, 34, 36, 41 and 48. The total number of tests conducted per year was 129, 135,123,116, 117, 135, 123, 133, 137 and 145.

Note: The number of tests conducted each year ranged from 116 to 145.

**Figure E3**. Geometric mean IC_{25} (percent effluent on a volume basis) and proportion of tests in each sublethal toxicity category per year for the plant growth inhibition test using *Lemna minor* dry weight

**Description**

Figure E3 is a line chart stacked above a proportional bar chart. The line chart illustrates the geometric mean IC_{25} per year from 2003 to 2012 for the plant growth inhibition test using *Lemna minor* dry weight. The geometric mean IC_{25} per year was 44, 33, 40, 50, 43, 49, 45, 45, 41 and 53. The bar chart illustrates the proportion of tests in three sublethal toxicity categories that include higher toxicity IC_{25} ≤20%, lower toxicity IC_{25}>20% and <100% and no toxicity IC_{25}≥100%. The number of tests in the higher category per year was 29, 46, 33, 23, 27, 23, 20, 27, 31 and 20. The number of tests in the lower category per year was 28, 31, 23, 19, 20, 21, 33, 32, 31 and 42. The number of tests in the no toxicity category per year was 63, 65, 69, 82, 70, 86, 69, 65, 73 and 71. The total number of tests conducted per year was 120, 142, 125, 124, 117, 130, 122, 124, 135 and 133.

Note: The number of tests conducted each year ranged from 117 to 142.

**Figure E4**. Geometric mean IC_{25} (percent effluent on a volume basis) and proportion of tests in each sublethal toxicity category each year for the alga growth inhibition test using *Pseudokirchneriella subcapitata*

**Description**

Figure E4 is a line chart stacked above a proportional bar chart. The line chart illustrates the geometric mean IC_{25} per year from 2003 to 2012 for the alga growth Inhibition test using *Pseudokirchneriella subcapitata*. The geometric mean IC_{25} per year was 55, 48, 53, 48, 59, 62, 69, 79, 81 and 64. The bar chart illustrates the proportion of tests in three sublethal toxicity categories that include higher toxicity IC_{25} ≤20%, lower toxicity IC_{25}>20% and <100% and no toxicity IC_{25}≥100%. The number of tests in the higher category per year was 16, 30, 22, 25, 16, 14, 8, 5, 2 and 13. The number of tests in the lower category per year was 42, 35, 42, 29, 29, 22, 19, 12, 16 and 22. The number of tests in the no toxicity category per year was 72, 80, 82, 77, 74, 97, 109, 121, 121 and 117. The total number of tests conducted per year was 130, 145, 146, 131, 119, 133, 136, 138, 139 and 152.

Note: The number of tests conducted each year ranged from 119 to 152.

**Figure E5**. Geometric mean IC_{25} (percent effluent on a volume basis) and proportion of tests in each sublethal toxicity category each year for the fish larval growth inhibition test using *Pimephales promelas*

**Description**

Figure E5 is a line chart stacked above a proportional bar chart. The line chart illustrates the geometric mean IC_{25} per year from 2003 to 2012 for the fish larval growth Inhibition test using *Pimephales promelas*. The geometric mean IC_{25} per year was 77, 76, 87, 89, 85, 89, 88, 90, 91 and 87. The bar chart illustrates the proportion of tests in three sublethal toxicity categories that include higher toxicity IC_{25} ≤20%, lower toxicity IC_{25}>20% and <100% and no toxicity IC_{25}≥100%. The number of tests in the higher category per year was 6, 4, 2, 2, 3, 1, 2, 2, 1 and 2. The number of tests in the lower category per year was 17, 34, 16, 9, 9, 12, 17, 16, 15 and 19. The number of tests in the no toxicity category per year was 103, 97, 120, 109, 99, 113, 108, 112, 116 and 119. The total number of tests conducted per year was 126, 135, 138, 120, 111, 126, 127, 130, 132 and 140.

Note: The number of tests conducted each year ranged from 111 to 140.

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