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Biological Test Method Series

Biological test methods and guidance documents are amended periodically through the publication of amendment sheets to be used in conjunction with existing methods, or through the publication of second editions. In recent documents, amendments have been highlighted in green.

This table describes biological test methods and guidance documents available via email. It provides a thumbnail image fo the report cover, lists each report title, publication date with amendments, and reference number.

Biological test methods and guidance documents

Report Cover	Report Title	Publication Date	Reference
	Biological Test Method: Acute Lethality Test Using Rainbow Trout	July 1990, Highlighted Amendments: May 1996, May 2007	EPS1/RM/09
	Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus)	July 1990, Amendments: March 2000	EPS1/RM/10
	Biological Test Method: Acute Lethality Test Using Daphnia spp.	July 1990, Amendments: May 1996	EPS1/RM/11
	Guidance Document on Control of Toxicity Test Precision Using Reference Toxicants	August 1990	EPS1/RM/12
	Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout	Second Edition: December 2000, Highlighted Amendments: May 2007	EPS1/RM/13
	Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magna	Second Edition: December 2000	EPS1/RM/14
	Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia	Second Edition Highlighted: February 2007	EPS1/RM/21
	Biological Test Method: Test of Larval Growth and Survival Using Fathead Minnows	Second Edition Highlighted: February 2011	EPS1/RM/22
	Biological Test Method: Toxicity Test Using Luminescent Bacteria	November 1992	EPS1/RM/24
	Biological Test Method: Growth Inhibition Test Using a Freshwater Alga	Second Edition Highlighted: March 2007	EPS1/RM/25
	Biological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine Amphipods	December 1992, Amendments: October 1998	EPS1/RM/26
	Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars)	Second Edition Highlighted: February 2011	EPS1/RM/27
	Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout)	Second Edition: July 1998	EPS1/RM/28
	Guidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological Testing	December 1994	EPS1/RM/29
	Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference Toxicant	September 1995	EPS1/RM/30
	Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius)	December 1997	EPS1/RM/32
	Biological Test Method: Test for Survival and Growth in Sediment and Water Using the Freshwater Amphipod Hyalella azteca	Second Edition - January 2013	EPS1/RM/33
	Guidance Document on Application and Interpretation of Single-species Tests in Environmental Toxicology	December 1999	EPS1/RM/34
	Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods	December 1998	EPS1/RM/35
	Biological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minor	Second Edition Highlighted: January 2007	EPS1/RM/37
	Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta)	December 2001	EPS1/RM/41
	Biological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase Test	April 2002	EPS1/RM/42
	Biological Test Method: Tests for Toxicity of Contaminated Soil to Earthworms (Eiseniaandrei,Eisenia fetida or Lumbricus terrestris)*	June 2004, Highlighted Amendments: June 2007	EPS1/RM/43
	Guidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial Organisms	March 2004	EPS1/RM/44
	Biological Test Method: Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in Soil*	February 2005, Highlighted Amendments: June 2007	EPS1/RM/45
	Guidance Document on Statistical Methods for Environmental Toxicity Tests*	March 2005,Highlighted Amendments: June 2007	EPS1/RM/46
	Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil* +	September 2007	EPS1/RM/47
	Procedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow Trout	March 2008	EPS1/RM/50
	Supplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent to Rainbow Trout	March 2008	N/A
	Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing	2012	EPS1/RM/53

* Only the English versions of these documents were subject to a June 2007 amendment as the changes were incorporated into the French documents before publication.

+ This PDF is text searchable (as with other files available here), with the exception of the following pages: 85, L-203, N-207.