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Biological Test Method Series

Biological test methods and guidance documents are amended periodically through the publication of amendment sheets to be used in conjunction with existing methods, or through the publication of subsequent editions. In recent documents, amendments have been highlighted in green.

This table describes biological test methods and guidance documents available via email. It provides a thumbnail image of the report cover and lists each report title, publication date with amendments, and reference number.

Biological Test Methods and Guidance Documents
Report CoverReport TitlePublication DateReference
 Cover of Biological Test Method: Acute Lethality Test Using Rainbow Trout

Biological Test Method: Acute Lethality Test Using Rainbow Trout

HTML Version 

July 1990, Highlighted Amendments: May 1996, May 2007EPS1/RM/09
 Cover of Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus)Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteusaculeatus)July 1990, Amendments: March 2000EPS1/RM/10
 Cover of Biological Test Method: Acute Lethality Test Using Daphnia spp.

Biological Test Method: Acute Lethality Test Using Daphnia spp.

HTML Version 

July 1990, Amendments: May 1996EPS1/RM/11
 Cover of Guidance Document on Control of Toxicity Test Precision Using Reference ToxicantsGuidance Document on Control of Toxicity Test Precision Using Reference ToxicantsAugust 1990EPS1/RM/12
 Cover of Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout

Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout

HTML Version 

Second Edition: December 2000, Highlighted Amendments: May 2007EPS1/RM/13
 Cover of Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magna

Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magna

HTML Version 

Second Edition: December 2000EPS1/RM/14
 Cover of Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia

Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia

HTML Version

Second Edition Highlighted: February 2007EPS1/RM/21
 Cover of Larval Growth and Survival Using Fathead Minnows

Biological Test Method: Test of Larval Growth and Survival Using Fathead Minnows

HTML Version

Second Edition Highlighted: February 2011EPS1/RM/22
 Cover of Biological Test Method: Toxicity Test Using Luminescent BacteriaBiological Test Method: Toxicity Test Using Luminescent BacteriaNovember 1992EPS1/RM/24
 Cover of Biological Test Method: Growth Inhibition using a Freshwater Alga

Biological Test Method: Growth Inhibition Test Using a Freshwater Alga

HTML Version

Second Edition Highlighted: March 2007EPS1/RM/25
 Cover of Biological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine AmphipodsBiological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine AmphipodsDecember 1992, Amendments: October 1998EPS1/RM/26
 Cover of Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars)

Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars)

HTML Version

Second Edition Highlighted: February 2011EPS1/RM/27
 Cover of Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout)

Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout)

HTML Version

Second Edition: July 1998EPS1/RM/28
 Cover of Guidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological TestingGuidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological TestingDecember 1994EPS1/RM/29
 Cover of Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference ToxicantGuidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference ToxicantSeptember 1995EPS1/RM/30
 Cover of Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius)Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius)December 1997EPS1/RM/32
 Cover of Biological Test Method: Test for Survival and Growth in Sediment and Water Using the Freshwater Amphipod Hyalella azteca.Biological Test Method: Test for Survival and Growth in Sediment and Water Using the Freshwater Amphipod Hyalella aztecaSecond Edition: January 2013EPS1/RM/33
 Cover of Guidance Document on Application and Interpretation of Single-species Tests in Environmental ToxicologyGuidance Document on Application and Interpretation of Single-species Tests in Environmental ToxicologyDecember 1999EPS1/RM/34
 Cover of Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods

Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods

HTML Version

December 1998EPS1/RM/35
 Cover of Biological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minorBiological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minorSecond Edition Highlighted: January 2007EPS1/RM/37
 Cover of Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta)Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta)December 2001EPS1/RM/41
 Cover of Biological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase TestBiological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase TestApril 2002EPS1/RM/42
 Cover of Tests for Toxicity of Contaminated Soil to Earthworms (Eisenia andrei, Eisenia fetida or Lumbricus terrestris)Biological Test Method: Tests for Toxicity of Contaminated Soil to Earthworms (Eiseniaandrei, Eisenia fetida or Lumbricus terrestris)Footnote 1June 2004, Highlighted Amendments: June 2007EPS1/RM/43
 Cover of Guidance document for testing the pathogenicity and toxicity of new microbial substances to aquatic and terrestrial organismsGuidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial OrganismsMarch 2004EPS1/RM/44
 Cover of Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in SoilBiological Test Method: Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in SoilFootnote 1February 2005, Highlighted Amendments: June 2007EPS1/RM/45
 Cover of Guidance Document on Statistical Methods for Environmental Toxicity TestsGuidance Document on Statistical Methods for Environmental Toxicity TestsFootnote 1March 2005, Highlighted Amendments: June 2007EPS1/RM/46
 Cover of Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil* +Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in SoilFootnote 1, Footnote 2September 2007EPS1/RM/47
 Cover of Procedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow TroutProcedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow TroutMarch 2008EPS1/RM/50
 Cover of Supplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent ot Rainbow TroutSupplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent to Rainbow TroutMarch 2008N/A
Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing2012EPS1/RM/53
Biological Test Method: Test for Growth in Contaminated Soil Using Terrestrial Plants Native to the Boreal Region Biological Test Method: Test for Growth in Contaminated Soil Using Terrestrial Plants Native to the Boreal Region2013EPS1/RM/56

Footnotes

Footnote 1

Only the English versions of these documents were subject to a June 2007 amendment, as the changes were incorporated into the French documents before publication.

Return to footnote 1

Footnote 2

This PDF is text searchable (as with other files available here), with the exception of the following pages: 85, L-203, N-207.

Return to footnote 2