Biological Test Method Series

Biological test methods and guidance documents are amended periodically through the publication of amendment sheets to be used in conjunction with existing methods, or through the publication of second editions. In recent documents, amendments have been highlighted in green.

Report CoverReport TitlePublication DateReference
 Cover of Biological Test Method: Acute Lethality Test Using Rainbow TroutBiological Test Method: Acute Lethality Test Using Rainbow TroutJuly 1990, Highlighted Amendments: May 1996, May 2007EPS1/RM/09
 Cover of Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus)Biological Test Method: Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus)July 1990, Amendments: March 2000EPS1/RM/10
 Cover of Biological Test Method: Acute Lethality Test Using Daphnia spp.Biological Test Method: Acute Lethality Test Using Daphnia spp.July 1990, Amendments: May 1996EPS1/RM/11
 Cover of Guidance Document on Control of Toxicity Test Precision Using Reference ToxicantsGuidance Document on Control of Toxicity Test Precision Using Reference ToxicantsAugust 1990EPS1/RM/12
 Cover of Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow TroutBiological Test Method: Reference Method for Determining Acute Lethality of Effluents to Rainbow TroutSecond Edition: December 2000, Highlighted Amendments: May 2007EPS1/RM/13
 Cover of Biological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magnaBiological Test Method: Reference Method for Determining Acute Lethality of Effluents to Daphnia magnaSecond Edition: December 2000EPS1/RM/14
 Cover of Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubiaBiological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubiaSecond Edition Highlighted: February 2007EPS1/RM/21
 Cover of Larval Growth and Survival Using Fathead MinnowsBiological Test Method: Test of Larval Growth and Survival Using Fathead MinnowsSecond Edition Highlighted: February 2011EPS1/RM/22
 Cover of Biological Test Method: Toxicity Test Using Luminescent BacteriaBiological Test Method: Toxicity Test Using Luminescent BacteriaNovember 1992EPS1/RM/24
 Cover of Biological Test Method: Growth Inhibition using a Freshwater AlgaBiological Test Method: Growth Inhibition Test Using a Freshwater AlgaSecond Edition Highlighted: March 2007EPS1/RM/25
 Cover of Biological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine AmphipodsBiological Test Method: Acute Test for Sediment Toxicity Using Marine or Estuarine AmphipodsDecember 1992, Amendments: October 1998EPS1/RM/26
 Cover of Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars)Biological Test Method: Fertilization Assay Using Echinoids (Sea Urchins and Sand Dollars)Second Edition Highlighted: February 2011EPS1/RM/27
 Cover of Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout)Biological Test Method: Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout)Second Edition: July 1998EPS1/RM/28
 Cover of Guidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological TestingGuidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological TestingDecember 1994EPS1/RM/29
 Cover of Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference ToxicantGuidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference ToxicantSeptember 1995EPS1/RM/30
 Cover of Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius)Biological Test Method: Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius)December 1997EPS1/RM/32
 Biological Test Method: Test for Survival and Growth in Sediment Using the Freshwater Amphipod Hyalella aztecaBiological Test Method: Test for Survival and Growth in Sediment Using the Freshwater Amphipod Hyalella aztecaDecember 1997EPS1/RM/33
 Cover of Guidance Document on Application and Interpretation of Single-species Tests in Environmental ToxicologyGuidance Document on Application and Interpretation of Single-species Tests in Environmental ToxicologyDecember 1999EPS1/RM/34
 Cover of Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine AmphipodsBiological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine AmphipodsDecember 1998EPS1/RM/35
 Cover of Biological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minorBiological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minorSecond Edition Highlighted: January 2007EPS1/RM/37
 Cover of Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta)Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta)December 2001EPS1/RM/41
 Cover of Biological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase TestBiological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase TestApril 2002EPS1/RM/42
 Cover of Tests for Toxicity of Contaminated Soil to Earthworms (Eisenia andrei, Eisenia fetida or Lumbricus terrestris)Biological Test Method: Tests for Toxicity of Contaminated Soil to Earthworms (Eisenia andrei,Eisenia fetida or Lumbricus terrestris)*June 2004, Highlighted Amendments: June 2007EPS1/RM/43
 Cover of Guidance document for testing the pathogenicity and toxicity of new microbial substances to aquatic and terrestrial organismsGuidance Document for Testing the Pathogenicity and Toxicity of New Microbial Substances to Aquatic and Terrestrial OrganismsMarch 2004EPS1/RM/44
 Cover of Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in SoilBiological Test Method: Test for Measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in Soil*February 2005, Highlighted Amendments: June 2007EPS1/RM/45
 Cover of Guidance Document on Statistical Methods for Environmental Toxicity TestsGuidance Document on Statistical Methods for Environmental Toxicity Tests*March 2005,Highlighted Amendments: June 2007EPS1/RM/46
 Cover of Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil* +Biological Test Method: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil* +September 2007EPS1/RM/47
 Cover of Procedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow TroutProcedure for pH Stabilization During the Testing of Acute Lethality of Wastewater Effluent to Rainbow TroutMarch 2008EPS1/RM/50
 Cover of Supplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent ot Rainbow TroutSupplementary Background and Guidance for Investigating Acute Lethality of Wastewater Effluent to Rainbow TroutMarch 2008N/A
Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing Guidance Document on the Sampling and Preparation of Contaminated Soil for Use in Biological Testing2012EPS1/RM/53

* Only the English versions of these documents were subject to a June 2007 amendment as the changes were incorporated into the French documents before publication.

+ This PDF is text searchable (as with other files available here), with the exception of the following pages: 85, L-203, N-207.