Draft Screening Assessment
Certain Organic Flame Retardants Substance Grouping
Dechlorane Plus (DP)
Chemical Abstracts Service Registry Number
Environment and Climate Change Canada
(PDF Format - 1120 KB)
Table of Contents
- 1. Introduction
- 2. Identity of Substances
- 3. Physical and Chemical Properties
- 4. Sources
- 5. Uses
- 6. Releases to the Environment
- 7. Measured Environmental Concentrations
- 8. Environmental Fate and Behaviour
- 9. Potential to Cause Ecological Harm
- 10. Potential to Cause Harm to Human Health
- 11. Conclusion
- 12. References
- 13. Appendices
- Appendix A: Structural Identity
- Appendix B: Physical-Chemical Properties
- Appendix C: Upper-bouding estimates of daily intake (μg/kg bw/d) of DP by various age groups within the general population of Canada
- Appendix D: Concentrations of DP in household dust
- Appendix E: Concentrations of DP in human breast milk
- Appendix F: Concentrations of DP in human serum
List of Tables and Figures
- Table 2-1. Substance identity for Decabromodiphenyl ethane
- Table 2-2. Analogue identity
- Table 3-1. A summary of the physical and chemical properties for DBDPE
- Table 5-1. Polymeric systems that may contain DP: Thermoplastics
- Table 5-2. Polymeric systems that may contain DP: Thermosets
- Table 7-1. Environmental Concentration Range, Canada
- Table 7-2. Environmental Concentration Range, Global
- Table 8-1. Results of the Level III fugacity modelling (New EQC 2012) for DP
- Table 8-2. Summary of key data for abiotic degradation of DP
- Table 8-3. Summary of key data for biodegradation of DP
- Table 8-4. Bioaccumulation factors (BAF) or Biota-Sediment Accumulation factors (BSAF) for DP
- Table 8-5. Empirical biomagnification factors (BMF) for DP
- Table 9-1. Calculated CBRs in fish for bioavailable DP
- Table 9-2. Key sediment toxicity studies considered in choosing a DP critical toxicity value for sediment (based on analogue chlordane)
- Table 9-3. Key soil toxicity studies considered in choosing a DP critical toxicity value for soil (includes analogues chlordane and mirex)
- Table 9-4. Key wildlife toxicity studies considered in choosing a DP critical toxicity value for soil
- Table 9-5. Summary of input values used for scenarios estimating aquatic concentrations resulting from industrial releases of DP
- Table 9-6. Risk quotients obtained for different media and exposure scenarios for DP
- Table 10-1. Total mortality of rats treated with DP in the RDT phase (Brock et al. 2010)
- Table 10-2. Total mortality of rats treated with DP in the DART phase (Brock et al. 2010)
- Table A-1. Other selected names for DP
- Table B-1. Physical-chemical value inputs, outputs, and percent adjustment for Least Squares Means Adjustment Model (LSA). Values in brackets are sub-cooled
- Table B-2. Physical and chemical properties for DP
- Table B-3. Summary of relevant physical-chemical properties for DP analogues: Chlordane and Mirex
Pursuant to section 68 of the Canadian Environmental Protection Act, 1999 (CEPA), the Ministers of the Environment and Climate Change and of Health have conducted a screening assessment of 1,4:7,10-dimethanodibenzo[a,e]cyclooctene, 1,2,3,4,7,8,9,10,13,13,14,14-dodecachloro-1,4,4a,5,6,6a,7,10,10a,11,12,12a-dodecahydro-, commonly known as Dechlorane Plus® (Dechlorane Plus or DP) (Chemical Abstracts Service Registry Number [CAS RN] 13560-89-9). DP is a substance within the Certain Organic Flame Retardants (OFR) Substance Grouping under Canada's Chemicals Management Plan, which includes ten organic substances having a similar function: the application to materials to slow the ignition and spread of fire. DP was identified as priority for assessment based on other human health concerns (i.e., based on intermediate potential for exposure to Canadians) but not for ecological concerns (met criteria for persistence but uncertain with respect to potential for bioaccumulation and inherent toxicity to non-human organisms).
DP does not occur naturally in the environment. Based on responses to a survey conducted under section 71 of CEPA, DP imports to Canada ranged from 1000 to 10 000 kg in 2011 for use as an additive flame retardant in several applications. Known international uses of DP include applications in wire and cable jacketing, electronics, appliances, automobiles, hard plastic connectors, and plastic roofing materials. DP has been produced for the last 40 years, although not in Canada, and is currently marketed as an alternative/replacement for Decabromodiphenyl ether (DecaBDE) in a range of flame retardant applications of electronic wiring and cables, automobiles, plastic roofing materials, and hard plastic connectors. DP is a High Production Volume substance in the USA, and manufacturing in China has recently been reported. Recent estimates of DP production range from 450 000 to 4 500 000 kg import/production in the USA.
DP release to the environment is most likely to occur during the manufacturing, formulation and/or industrial use stages of these sectors. Releases to the environment are expected to occur primarily through wastewater, with some release to water directly from industrial sites. Although DP can be found in consumer or commercial products, information on releases to the environment from this route is limited, and releases are expected to be diffuse and low relative to industrial releases. Generally, DP is characterized by very low water solubility, low to very low vapour pressure, and a very high organic carbon-water partition coefficient and octanol-water partition coefficient. When released to the environment, DP is expected to predominantly reside in soil and/or sediment, depending on the compartment of release, with less than 4% remaining in air or water. Based on some detection of DP in remote Arctic areas, and a possibly high predicted transfer efficiency (OECD POPs model), particle-bound transport may be important for long-range transport of this substance. DP has been measured in the Canadian environment, as well as internationally, in most media.
Experimental and modelled data indicate that aerobic and anaerobic biodegradation of DP is very limited and that DP is expected to be highly persistent in water, soil, and sediment. Modelled predictions for DP in air suggest a half-life less than 1 day for the gas phase, but DP is most likely to be sorbed to airborne particulates, and therefore persistence in air could be longer.
Published bioaccumulation and biomagnification studies, as well as widespread measurements in biota, indicate that DP may be highly bioaccumulative and may biomagnify in organisms and food webs.
Given the limited empirical aquatic toxicity data for DP (due to low solubility in water), the toxicity potential in fish from dietary uptake in water was investigated using a Critical Body Residue (CBR) approach. CBR results suggest DP in biota (Canadian fish tissue) does not reach tissue concentration resulting in acute or chronic lethality in aquatic organisms. Due to a lack of soil and sediment ecotoxicity data for DP, chronic toxicity data for two analogue substances, chlordane (CAS RN 57-74-9) and mirex (CAS RN 2385-85-5), were evaluated. Although these analogues are considered conservative, results suggest that DP can cause effects at low concentrations in sediment and soil organisms.
Industrial scenarios were developed to provide estimates of exposure, based on available industrial site information including potential quantities used. These scenarios involved: industrial wastewater release to surface water resulting in DP partitioning to sediment; and DP in wastewater partitioning to biosolids, followed by biosolids application to soil. Risk quotient analyses, integrating conservative estimates of exposure with toxicity information, were performed for sediment and soil organisms, and wildlife. Results of these analyses indicate that although in most scenarios DP poses a low risk to organisms based on current levels of use and release in Canada, at least one soil exposure scenario suggests predicted environmental concentrations of DP approach a level that could result in risk to soil organisms (i.e. risk quotient = 0.78).
DP's high persistence suggests the potential for build-up in the environment from past and current emissions, resulting in long-term exposures in sediment and soil. DP is expected to strongly adsorb to suspended solids/particulates when released to surface water, either directly from industrial activities or indirectly via wastewater treatment systems, and eventually settle in depositional sediment areas (i.e. sinks). Several studies have reported DP sediment concentrations in the Great Lakes region that exceed the predicted environmental concentrations for sediment developed from industrial scenarios based on quantities in use in Canada, suggesting that DP exposure in specific areas of Canada could be underestimated and precaution is warranted. Risk quotient analyses that compare these upper measured sediment values vs. the sediment PNEC could result in values greater than 1, indicating risk to sediment-dwelling organisms. It should be noted that DP is a High Production Volume substance in the USA; past and/or present environmental transport of DP from the northern USA, in particular manufacturing near the Great Lakes, may therefore contribute to DP exposure in Canada.
Considering all available lines of evidence presented in this draft screening assessment, there is risk of harm to organisms, but not to the broader integrity of the environment from DP. It is proposed to conclude that DP meets the criteria under paragraph 64(a) of CEPA as it is entering or may enter the environment in a quantity or concentration or under conditions that have or may have an immediate or long-term harmful effect on the environment or its biological diversity. However, it is proposed to conclude that DP does not meet the criteria under paragraph 64(b) of CEPA as it is not entering the environment in a quantity or concentration or under conditions that constitute or may constitute a danger to the environment on which life depends.
No classifications of the health effects of DP by national or international regulatory agencies were identified. Based on the available information on genotoxicity, DP is considered unlikely to be genotoxic. In repeated-dose oral toxicity studies, no adverse effects were observed up to the highest dose level tested in animal studies.
The main sources of exposure for the general population in Canada are expected to be from environmental media (air, dust, soil, and water), and food, including breast milk. International and Canadian biomonitoring data are available.
There are eight orders of magnitude between estimates of exposure from environmental media and food and the highest dose level tested. These margins are considered to be adequate to account for uncertainties in the exposure and health effect databases. Therefore, it is proposed to conclude that DP does not meet the criteria under paragraph 64(c) of CEPA, as it is not entering the environment in a quantity or concentration or under conditions that constitute or may constitute a danger in Canada to human life or health.
Overall Proposed Conclusion
It is proposed to conclude that DP meets one or more of the criteria set out in section 64 of CEPA. DP is proposed to meet the persistence and bioaccumulation criteria as set out in the Persistence and Bioaccumulation Regulations of CEPA.
Pursuant to sections 68 and 74 of the Canadian Environmental Protection Act, 1999 (CEPA), the Minister of the Environment and Climate Change and the Minister of Health conduct screening assessments of substances to determine whether these substances present or may present a risk to the environment or to human health.
The Substance Groupings Initiative is a key element of the Government of Canada's Chemicals Management Plan. The Certain Organic Flame Retardants Substance Grouping consists of ten substances identified as priorities for assessment as they met the categorization criteria under section 73(1) of CEPA, and/or were considered as a priority based on ecological and/or human health concerns (Environment and Climate Change Canada and Health Canada 2007). All of these substances have a similar function: the application to materials to slow the ignition and spread of fire. These substances are also potential alternatives for other flame retardants which are presently subject to regulatory controls or phase-out in Canada and/or globally.
This screening assessment focuses on the substance 1,4:7,10-Dimethanodibenzo[a,e]cyclooctene, 1,2,3,4,7,8,9,10,13,13,14,14-dodecachloro-1,4,4a,5,6,6a,7,10,10a,11,12,12a-dodecahydro-, commonly known as Dechlorane Plus® (Dechlorane Plus or DP). The Chemical Abstracts Service Registry Number (CAS RN) for DP is 13560-89-9.
This substance was identified in the categorization of the Domestic Substances List (DSL) under subsection 73(1) of CEPA as a priority for assessment based on other human health concerns. DP met criteria for persistence, but was uncertain with respect to meeting criteria for inherent toxicity to nonhumans and bioaccumulation.
Screening assessments focus on information critical to determining whether substances within a grouping meet the criteria as set out in section 64 of CEPA, by examining scientific information to develop conclusions by incorporating a weight-of-evidence approach and precaution.Footnote 1
This draft screening assessment includes consideration of information on chemical properties, environmental fate, hazards, uses and exposure, including additional information submitted by stakeholders. Relevant data were identified up to August 2014 for the ecological assessment and for the human health assessment. However, a cursory search was conducted to include any salient literature up to July 2015. Empirical data from key studies as well as some results from models were used to reach proposed conclusions. When available and relevant, information presented in assessments from other jurisdictions was considered.
The draft screening assessment does not represent an exhaustive or critical review of all available data. Rather, it presents the most critical studies and lines of evidence pertinent to the proposed conclusion.
This draft screening assessment was prepared by staff in the Existing Substances Programs at Health Canada and Environment and Climate Change Canada and incorporates input from other programs within these departments. The ecological and human health portions of this assessment have undergone external written peer review and/or consultation. Comments on the technical portions relevant to the environment were received from Jon Arnot (Arnot Research and Consulting), Li Shen (Ontario Ministry of the Environment), and Ian Doyle (UK Environment Agency). Comments on the technical portions relevant to human health in this draft screening assessment were received from scientific experts selected and directed by Toxicology Excellence for Risk Assessment (TERA). Comments were received from Patricia McGinnis (Independent Consultant), Pam Williams (E Risk Sciences) and Paul Rumsby (National Centre for Environmental Toxicology). While external comments were taken into consideration, the final content and outcome of the screening assessment remain the responsibility of Health Canada and Environment and Climate Change Canada.
The critical information and considerations upon which the draft assessment is based are summarized below.
2. Substance Identity
This screening assessment focuses on 1,4:7,10-Dimethanodibenzo[a,e]cyclooctene, 1,2,3,4,7,8,9,10,13,13,14,14-dodecachloro-1,4,4a,5,6,6a,7,10,10a,11,12,12a-dodecahydro- (Dechlorane Plus® or DP). This substance is an organic flame retardant within the Certain Organic Flame Retardants (OFRs) Substance Grouping under the Substance Groupings Initiative of the CMP. The structural identity of this substance is presented in Table 2 1. Other names for the substance are presented in Appendix A. For this assessment, Dechlorane Plus will be referred to as DP.
The commercial technical product DP is primarily a mixture of the syn and anti stereoisomers, typically composed of approximately 25% syn-DP and 75% anti-DP (Sverko et al. 2011). DP is a chlorinated cycloaliphatic flame retardant produced by a Diels-Alder condensation of 1,5-cyclooctadiene and hexachlorocyclopentadiene in a 2:1 molar ratio (Sverko et al. 2011).
|CAS RN||Chemical structure||Molecular mass||Chemical formula|
2.1 Selection of Analogues and Use of (Q)SAR Models
Guidance on the use of a read-across approach and Quantitative Structure-Activity Relationships or (Q)SAR models for filling data gaps has been prepared by various organizations such as the Organisation for Economic Co-operation and Development (OECD). These methods have been applied in various regulatory programs including the European Union's Existing Substances Programme. In this assessment, a read-across approach using data from analogues and the results of (Q)SAR models, where appropriate, has been used to inform the ecological assessment.
Analogues were selected that were structurally similar and functionally similar to DP (e.g., based on sediment and soil toxicity), and that had relevant empirical data that could be used to read-across. The applicability of (Q)SAR models was determined on a case-by-case basis. Details of the read-across data and (Q)SAR models chosen to inform the ecological and human health assessments of DP are further discussed in the relevant sections of this report.
The analogues used to inform the sediment and soil toxicity sections of this ecological assessment are presented in Table 2-2 . There are limited analogue options for DP, given that the most similar chemical analogues to DP also lack ecotoxicity data for soil and sediment. DP is a replacement for the flame retardant use of organochlorine mirex (also called Dechlorane, CAS RN 2385-85-5) (Feo et al. 2012); therefore mirex was identified as a potential analogue. Chlordane was also identified by the OECD (Q)SAR Application Toolbox as a structurally and functionally close analogue for which sediment and soil toxicity data were available. DP, chlordane, and mirex (as well as other "dechloranes") are all similarly synthesized from hexachloro-cyclopentadiene and are expected to behave similarly in the environment (e.g., partitioning to soil and sediment, stable/persistent etc.) (Environment and Climate Change Canada 1977). Using chlordane and mirex as analogues for toxicity is conservative, as they are more bioavailable and therefore likely more toxic than DP (at least to aquatic organisms) due to higher water solubility than DP. As a result, these analogues were considered "worst-case" and protective in relation to ecological effects for sediment and soil organisms.
Although mirex was never registered for use as a pesticide in Canada, it has been used worldwide as a stomach insecticide for ant and other insect pests, and as a flame retardant (Environment and Climate Change Canada 2014, IPCS 1984, IPCS 1988). Chlordane is an organochlorine pesticide that was used in Canada from the mid-1940s to the1980s, but its registration and use under the Pest Control Products Act were discontinued as of 1991 (CCME 1999). Both chlordane and mirex appear on the List of Toxic Substances (Schedule 1) of CEPA.
Other "dechlorane-related" substances, such as dechlorane 602 (CAS RN 31107-44-5), dechlorane 603 (CAS RN 13560-92-4), dechlorane 604 (CAS RN 34571-16-9), and Chlordene Plus (CAS RN 13560-91-3) are known analogues of DP (Sverko et al. 2011), and are also detected in the environment and biota. Dechlorane 602, 603, and 604 are identified as flame retardants themselves, and Dechlorane 603 and Chlordene Plus are detected in organochlorine pesticides (Shen et al. 2011a). Dechlorane 602 and Dechlorane 604 are listed on Canada's Non-Domestic Substance List (NDSL), indicating they are in use internationally; however, it is expected that any use of the four substances in Canada would be low. Furthermore, these substances have limited or no data with respect to sediment and soil toxicity, and as a result have no relevant empirical data for use in read across in the DP ecological assessment. Finally, DP-related compounds also include impurities formed through side reactions in DP synthesis (e.g., 1,4-DP, Vinalcyclohexane (VCH)-DP, 1,3- DP Monoadduct(DPMA), 1,5-DPMA) (Sverko et al. 2010). These compounds were measured in sediment core in Niagara River downstream of a USA DP industrial producer (Sverko et al. 2010). Assessment of these "dechlorane-related" substances is considered to be beyond the scope of this screening assessment.
|Substance CAS RN||Substance name||Molecular Weight (g/mol)||Empirical Structure/|
(Dodecachloro-pentacyclo [5.3.0.02,6.03,9.04,8] decane)
For the human health risk assessment, no appropriate analogues were identified for filling data gaps and a (Q)SAR approach was used to predict the potential for DP to be carcinogenic. Further details are provided in the Health Effects Assessment section.
3. Physical and Chemical Properties
Physical and chemical properties determine the overall characteristics of a substance and are used to determine the suitability of different substances for different types of applications. Such properties also play a critical role in determining the environmental fate of substances (including their potential for long-range transport), as well as their toxicity to humans and non-human organisms. A summary of experimental, modelled, and key values for the physical and chemical properties of DP that are relevant to its environmental fate and ecotoxicity can be found in Table 3-1 . A detailed table of physical and chemical properties of DP (empirical and modelled) and a summary of analogue physical and chemical properties can be found in Appendix B.
DP was considered amenable to model prediction of physical-chemical properties using (Q)SARs, as it is within the model domain of applicability (i.e., structural and/or property parameter domains are represented in the training set used for the models). Physical-chemical properties of DP were checked for internal consistency according to the Least-Squares Adjustment Procedure (LSA) (Schenker et al. 2005). Geometric mean or arithmetic mean values (for log values) of the most reliable and independent values found from empirical data and modelling were used to determine inputs to the LSA (sub-cooled values for water solubility, vapour pressure, and octanol solubility were used) (Table B-1; for all Physical-Chemical values see Table B-2). In determining internal consistency of the properties, the LSA model also produces predicted values. For the purposes of this assessment, the log Kow value 8.78, derived from the LSA method, was selected. This value is on the low end of the range of model predicted values, and is therefore conservative for estimates of bioaccumulation. To maintain internal consistency of physical chemical values, the LSA method value for water solubility and vapour pressure were also considered. The LSA water solubility value (2.85 x 10-7 mg/L) falls within the range of model predicted values (Table 3-1 ), but is slightly lower than reported empirical estimates. As the original water solubility test report details and methods are not available to the Government of Canada for review, this assessment uses the LSA water solubility value for modelling purposes. Final selected values are summarized in Table 3-1 (references are provided in Appendix B).
Generally, DP is characterized by very low water solubility, low to very low vapour pressure, and a very high organic carbon-water partition coefficient and octanol-water partition coefficient.
|Property||Experimental/Estimate||Modelled||Selected Value for Modelling|
|Melting point (°C)||greater than 325 - 350||170 - 350||350|
|Boiling point (°C)||N/A- degrade before boiling||486.83||N/A|
|Vapour pressure (Pa)||0.8 (at 200°C)||3.57 x 10-11 - 1.01 x 10-8||6.57 x10-11|
(1.08 x 10-7
|Henry's Law constant (Pa·m3/mol)||NA||0.151 to 0.754||0.151|
|Log Kow||NA||8.29 -11.27||8.78|
|Log Koc||6.65||7.62 - 7.68||6.65|
|Log Koa||NA||12.99 - 14.79||12.99|
|less than 1.67 x10-6 - 2.49 x 10-4||4.42 x 10-10 - 8.4 x 10-4||2.85 x 10-7|
(4.69 x 10-4 subcooled liquid)
aDetailed physical-chemical property values and references are provided in Appendix B.
There are no known natural sources of DP; DP is manufactured and sources of DP are anthropogenic. Currently there are two known producers of DP in the world: one located in the U.S. and the other in China (Hoh et al. 2006; Wang et al. 2010). The worldwide annual production volume for DP has been estimated at approximately 4500-5000 tonnes (Wang et al. 2010, Feo et al. 2012).
Based on a survey conducted for the year 2011 under section 71 of CEPA, between 1 to 10 tonnes of DP, including DP in some products and/or manufactured items, were imported into Canada by a few companies. No DP was identified as being manufactured in Canada for this year (Canada 2013). According to the result of a DSL Inventory Update conducted for the year 2008, DP was found to be imported into Canada by a number of companies in similar quantities (same order of magnitude range) as reported in 2011.
In the United States, DP is a High Production Volume chemical (US EPA 2011). According to the US EPA, DP production/import quantities in the U.S. have been constant within the same reporting range of 450-4500 tonnes for 1986-2006.
A manufacturer in China has been producing DP since approximately 2003-2005, with annual DP production estimated to range from 300 to 1000 tonnes (Wang et al. 2010).
Internationally, DP is used as an additive flame retardant in applications of electronic wiring and cables, automobiles, plastic roofing materials, and hard plastic connectors (Weil and Levchik 2009, Sverko et al. 2008, Sverko et al. 2010, Sverko et al. 2011, ECHA 2013), and similar uses of DP are expected in Canada. According to submissions under section 71 of CEPA, DP is used in Canada as a flame retardant in automobile manufacturing (ECCC 2013-2014).
As a flame retardant, DP is used in many polymeric systems. These systems are typically either thermoplastics or thermosets, as seen in Table 5-1 and 5-2. Thermoplastics have a reversible curing process whereas thermosets have an irreversible curing process (Modor Plastics 2013). Examples of thermoplastics that may contain DP include nylon (Weil and Levchik 2009), polyester (KEMI 2007), acrylonitrile butadiene styrene (ABS), natural rubber, polybutylene terephthalate (PBT), polypropylene, and styrene butadiene rubber (SBR) block copolymer (OxyChem 2007). DP may be used in thermosets such as epoxy and polyester resins, polyurethane foam, polyethylene, ethylene propylene diene monomer rubber, polyurethane rubber, silicon rubber, and neoprene (OxyChem 2007). The amount of DP in these materials ranges from 8% in PBT up to 40 % in silicon rubber (OxyChem 2007). According to manufacturer literature (OxyChem 2007), DP is manufactured for use solely by industrial customers. DP is an additive flame retardant in primary industrial applications.
|Product Type||DP Concentration||Reference|
|Nylon||0 – 35%||KEMI 2007; Weil and Levchik 2009|
|Polyester||0 – 16%||KEMI 2007|
|Acrylonitrile butadiene styrene||16.9%||OxyChem 2007|
|Natural Rubber||18.7%||OxyChem 2007|
|Polybutylene terephthalate (PBT)||8 – 18%||OxyChem 2007|
|Polypropylene||20 – 35%||OxyChem 2007|
|Styrene butadiene Rubber (SBR) Block Copolymer||30%||OxyChem 2007|
|Product Type||DP Concentration||Reference|
|Epoxy Resins||25.5%||OxyChem 2007|
|Unsaturated Polyester Resins||NS||OxyChem 2007|
|Polyurethane foam||17.5 – 35%||OxyChem 2007|
|Cross-linked Polyethylene||25.5%||OxyChem 2007|
|Polyurethane Rubber||20 – 30%||OxyChem 2007|
|Silicon Rubber||18.8 – 40%||OxyChem 2007|
|Ethylene propylene diene monomer rubber (EPDM)||33%||OxyChem 2007|
Abbreviations: NS = not specified
The applications and the product types in which the above polymer materials are found are mainly related to electrical and electronic applications such as electrical wire coatings, coil bobbins, hard plastic computer and TV connectors, switches, cable straps, power tool housing, and wall plates (Weil and Levchik 2009). DP may also be used as a flame retardant in military textiles; however, this use is minor (Weil and Levchik 2009). Furthermore, ECHA (2013) lists DP as being used in leather articles.
DP may also be used in epoxy resins and phenolic laminates and resins (OxyChem 2007), although their applications in consumer products are not known.
DP is not listed as an approved food additive in the Lists of Permitted Food Additives as regulated under the Food and Drugs Act, nor has it been identified as being used/present in formulations of food packaging materials or incidental additives (Health Canada 2013; 2013 email from Food Directorate, Health Canada, to Risk Management Bureau, Health Canada; unreferenced). DP is not listed in the Drug Products Database, the Therapeutic Products Directorate's internal Non-Medicinal Ingredient Database, the Natural Health Products Ingredients Database or the Licensed Natural Health Products Database as a medicinal or non-medicinal ingredient present in final pharmaceutical products, natural health products or veterinary drugs in Canada (DPD 2013, NHPID 2013, LNHPD 2013; 2013 email from the Therapeutic Products Directorate, Health Canada, to Risk Management Bureau, Health Canada; unreferenced). Based on notifications submitted under the Cosmetic Regulations to Health Canada, DP is not used in cosmetic products in Canada (2014 emails from the Consumer Product Safety Directorate, Health Canada, to the Existing Substances Risk Assessment Bureau, Health Canada; unreferenced).
6. Releases to the Environment
Anthropogenic releases to the environment depend upon various losses occurring during the manufacture, industrial use, consumer/commercial use, service life, and disposal of a substance and products containing that substance. Releases of DP to the Canadian environment, due to the substance's use as a flame retardant, are expected to be diffuse with some point sources (e.g., from processing facilities). Additive use of DP in products suggests diffuse emissions may occur from consumer or commercial products, and although there are uncertainties, the rate is assumed to be low in comparison to industrial point sources during incorporation of DP into products. Overall, releases from products are expected to be geographically dispersed and spread out over the duration of the service life and end-of-life of these products.
Although DP is identified with low usage in Canada, the substance is a High Production Volume substance in the USA, and is manufactured in the Great Lakes region (Niagara Falls, New York). Within Canada, many studies have measured relatively high DP concentrations in media in the Great Lakes region, particularly in the vicinity of the Niagara River and Lake Ontario, downstream of the DP manufacturing facility, suggesting release of DP to the environment from manufacturing activities (Hoh et al. 2006, Sverko et al. 2010).
Releases from industrial activities could also be expected to occur during cleaning of empty transport containers. Releases to the environment are expected to occur primarily through wastewater. Release to the soil could also occur through the application of biosolids to agricultural and pasture lands.
Although DP has low volatility, emissions to air (e.g., from industrial manufacturing release to air, dust, or release from products) can result in atmospheric deposition to soil and water (Sverko et al. 2010). When a substance is unintentionally transferred to land, it may be washed into the sewer or surface water or transferred by wind or rain to nearby soil. However, due to the low volatility of DP, this pathway of release is expected to be very limited. Finally, landfills that do not collect and treat their leachate may potentially release substances to ground or surface water via leachate. Furthermore, although limited, there is potential for releases of substances to the atmosphere from landfills that do not collect and destroy their landfill gas.
This information is used to further develop exposure characterization scenarios to estimate resulting environmental concentrations.
7. Measured Environmental Concentrations
DP has been measured in the Canadian environment, as well as globally, with the highest concentrations found close to urban or industrial areas. Within Canada, many studies have measured relatively high DP concentrations in media in the Great Lakes region, particularly in the vicinity of the Niagara River and Lake Ontario, downstream of an American DP manufacturing facility. For specific study results and details (including detection limits, sample size etc.), see supporting documentation (Environment and Climate Change Canada 2015). For the simplified range of concentrations measured in Canada and globally, see Table 7-1 and Table 7-2.
As part of the Integrated Atmospheric Deposition Network (IADN), several studies have measured DP air concentrations in the Great Lakes region (Canada and USA) of North America (Hoh et al. 2006, Venier and Hites 2008, Salamova and Hites 2011). Between 2005 and 2010, total concentrations from two remote, one rural and two urban sites ranged from 0.17- 2.5 pg/m3 in the vapour phase, while concentrations in the particulate phase were much greater and ranged from not detected to 340.36 pg/m3 (Salamova and Hites 2011, Hung et al. 2014, unpublished data). The anti-isomer almost always exceeded the syn-isomer in both the vapour phase and particulate phase. The highest measurements (e.g., 490 pg/m3) were observed at a sampling site located in Sturgeon Point, New York, 50 km from a DP production facility (Hoh et al. 2006). A significant decrease in DP concentrations was observed with increasing distance from the DP production facility (Sverko et al. 2011).
Recent high-volume active air sampling at a semi-urban location in Toronto, Canada, determined DP was one of the most frequently detected OFRs (87-96% detection frequency), with total (gas and particulate) DP concentrations up to 2.3 pg/m3syn-DP, and up to 5.2 anti-DP pg/m3(Shoeib et al. 2014).
Xiao et al. (2012) detected DP in 11 out of 14 high-volume air samples taken at a remote station in Alert in Nunavut, Canada. Total DP concentrations ranged from not detected (less than 0.05 pg/m3) to 2.1 pg/m3. Average syn-DP and anti-DP concentrations were 0.18 and 0.57 pg/m3, respectively, with the dominant isomer being anti-DP.
Concentrations of DP in precipitation have been measured in the Great Lakes region (50 - 890 pg/L) (Salamova and Hites 2011). Concentrations of DP were measured in the surface waters of two remote lakes (Lake Opeongo and Lake Sisikiwit) within the Canadian Shield region, as well as two of the lower Great Lakes, between 2005 and 2010 (Muir et al. 2011). The highest concentrations were measured in Lake Ontario at 6.7 pg/L, followed by Lake Opeongo and Lake Erie at 2.4 pg/L and 1.7 pg/L, respectively. Samples collected from Lake Siskiwit fell below the studies limit of detection. Venier et al. (2014) sampled Great Lakes surface water and reported highest DP in Lake Ontario (14 pg/L) and the lowest in Lake Huron (1.0 pg/L). Muir et al. (2014) reported a mean DP measurement of 4.89 pg/L from the central waters of Lake Ontario.
No soil measurements for DP have been reported for Canada. However, DP soil concentrations as high as 13 400 ng/g dry weight (dw) have been reported in Hui'an, China (Wang et al. 2010).
Numerous studies have quantified DP in surficial sediments of the North American Great Lakes region. A comprehensive study by Shen et al. (2010) measured DP in the surficial sediments of Lake Ontario (sampling sites in both the USA and Canada), Lake Erie, Lake Michigan, Lake Superior, and Lake Huron, and found syn-DP and anti-DP ranging from 0.0048- 23 μg/kg dw and 0.009-82 μg/kg dw, respectively (total DP ranged from 0.014 to 110 μg/kg dw). For both isomers, the average lowest concentrations of DP in surficial sediment were in Lake Superior (although the lowest single site was located in Lake Huron) and the highest concentrations were in Lake Ontario, which is located downstream from the DP manufacturing plant in Niagara Falls, New York (Sverko et al. 2011). Shen et al. (2011b) measured DP in surficial sediment Canadian tributaries to the Great Lakes, and measured the highest DP in Niagara River sediments (sampling sites in both USA and Canada): 21 to 310 μg/kg dw. Sverko et al. (2008) reported total DP concentrations ranging from 2.23-586 μg/kg dw for surficial sediment concentrations in Lake Ontario in samples collected in 1998. Sverko et al. (2008) also reported a range of 0.061-8.62 μg/kg dw for Lake Erie samples from 1997 and 1998.
Sediment cores taken from the Great Lakes have been used to examine changes in DP concentrations over time. Sverko et al. (2010) report the highest observed concentration of DP as 920 μg/kg dw in a core section corresponding to Lake Ontario during 1976-1980, near the mouth of the Niagara River. This study reported decreases of DP after this peak, coinciding with USA federal and state laws to mitigate free release of chemicals (such as DP) to the Niagara River (including installation of a water treatment plant; Sverko et al. 2010). Sediment core studies by Qiu et al. (2007) and Shen et al. (2010, 2011a) in Lake Ontario found a consistent time period: DP concentrations in sediment peaked in the early 1980s, and depending on location within Lake Ontario, have fluctuated around two-thirds of the maximum or decreased slightly since peak concentrations. Sverko et al. (2008) collected sediment cores at different locations within Lake Erie in order to compare concentrations between 1980 and 2002. The study found concentrations to decrease spatially westward with highest average concentrations of 40 μg/kg dw and 2.5 μg/kg dw in the east and west basin respectively. Yang et al. (2011) collected 16 sediment cores in the Great Lakes and found a maximum DP concentration in Lake Ontario 183 times greater than in the other lakes. Yang et al. (2011) also determined that although fluxes of DP to Lake Ontario have declined from the peak of 14-20 ng/cm2 /y in the 1990s, recent fluxes to Lake Ontario are 2-4 ng/cm2 /y. As DP is still being produced at present, the decline of input to Lake Ontario may reflect the decrease in production volume or an improvement in controlling the discharge (Yang et al. 2011). In contrast to Lake Ontario, in Lake Superior, the rate of DP input is increasing at all sites except one (Yang et al. 2011).
DP has been quantified in suspended sediments of the Niagara River through biweekly sampling studies by Shen et al. (2011a), Sverko et al (2008), Reiner et al. (2006). Concentrations ranged from 5.4 to 89 μg/kg dw between 1980 and 2002. A half-life in suspended sediment in the Niagara River was determined as 17 years (Reiner et al. 2006).
The only Canadian sediment DP samples not from the Great Lakes are from Lake Winnipeg, Manitoba, collected between 2000 and 2003. These showed mean concentrations of 0.0117 and 0.0183 μg/kg dw for syn-DP and anti-DP, respectively (Tomy et al. 2007).
Few studies in the literature report on Canadian or North American DP concentrations in wastewater effluent and wastewater treatment system by-products (e.g., sludge/biosolids). In a study conducted by Kolic et al. (2009), both syn-DP and anti-DP were detected in sludge collected from a Toronto area wastewater treatment plant. Concentrations were approximately 100 ng/g and from 10 to 100 ng/g for syn-DP and anti-DP respectively (values read from log-scale graph).
Many studies have quantified DP in biota sampled in North America, particularly in the Great Lakes region. For example, Muir et al. (2011, 2014) measured concentrations of DP in zooplankton, mysids, forage fish, and lake trout samples collected from Lake Erie, Lake Ontario, and Lake Opeongo between 2005 and 2010. Concentrations ranged from below detection to 0.070 ng/g ww, with highest concentrations in sculpin from Lake Ontario. DP temporal trends have been studied in the Great Lakes via analysis of tissue samples of lake trout, a top predator. Samples were collected every four to six years in Lake Ontario from 1979 to 2004, and reported DP tissue concentrations ranged from 0.31-0.85 ng/g ww (2.3-7.2 ng/g lw) (Ismail et al. 2009) and 0.2-1.6 ng/g ww (1.3-11 ng/g lw) (Reiner et al. 2006), with half-lives of 16 and 14 years, respectively. Shen et al. (2010) reported 20-440 pg/g lw for syn-DP and 33-330 pg/g lw for anti-DP for lake trout samples from 1998 and 2002 from Lake Superior, Lake Huron and Lake Ontario. DP has been measured in several other fish species in Canada as well (Hoh et al. 2006; Tomy et al. 2007; Houde et al. 2014, etc.)
DP has been measured in birds in Canada, including Peregrine Falcon eggs and Herring Gull eggs (DP:1.5 to 4.5 ng/g ww), collected from the Great Lakes, with the highest concentrations identified in the Niagra River colony or nests on Lake Ontario, closest to the DP manufacturing plant (Gauthier et al. 2007, Guerra et al 2011). Venier et al. (2010) quantified DP in the plasma of bald eagles in Canada (mean DP=0.19 ng/g ww).
Limited Canadian terrestrial data are available; however, Muir et al. (2014) report Arctic DP data for wolf tissue.
For further details on other Canadian biota studies, see supporting documentation (Environment and Climate Change Canada 2015).
|Media||Location(s)||Years (not continuous)||Concentration Range|
|Air (pg/m3)||Ontario, Nunavut||2004-2010||less than 0.05 - 340.36|
|Water (pg/L)||Lake Erie, Lake Ontario, Lake Opeongo, Lake Siskiwit||2005-2010||less than DL - 950 ±190 (precipitation)|
less than DL - 14.2 (lake water)
|Sediment (μg/kg dw)||Lake Superior, Lake Huron, St. Clair river, Lake Erie, Niagara River, Lake Ontario, Lake Winnipeg||~1975-2007||less than DL - 310 (2003)|
2.23 - 586 (1998)
920 (max. value, 1976 - 1980)
|Biosolids, sludge (ng/g)||Toronto, Canada||NS||Detected.|
Read from Graph:
10 - 100
|Biota - aquatic (pg/g lw)||Lake Erie, Lake Ontario, Lake Opeongo, Lake Winnipeg, Lake Superior, Lake Huron, Lake Erie, Niagara River||1979-2010||less than DL - 2600|
|Biota - terrestrial and avian (ng/g lw)||Northwest Territories, Niagara Falls, Canadian Great Lake Basin, Whistler,BC||2000-2010||less than DL - 230|
Abbreviations: DL = detection limit.
a See supporting documentation (Environment and Climate Change Canada 2015) for references and study details.
b Although wastewater system effluent and sludge/biosolids are not "environment," they represent a direct source to the environment and are included in this table.
|Media||Location(s)||Years (not continuous)||Concentration Range|
|Air (pg/m3)||Canada, U.S.A., Denmark, Norway, Sweden, Spain, China, North Sea, South Korea, Mongolia, Pacific Ocean, Indian Ocean, Southern Ocean, Arctic-Antarctica||2004-2011||less than DL - 26 734|
|Water (pg/L)||Canada, U.S.A., North Sea, China, Japan, Arctic-Antarctica||1974-2010||less than DL - 1 740|
|Sediment (ng/g dw)||Canada, U.S.A., Great Lakes, Denmark, Faroe Islands, Finland, Norway, Sweden, Spain, China||~1975-2011||Syn-DP:|
less than DL - 720
Anti-DP: less than DL - 2640
|Soil (ng/g dw)||China||2006-2010||less than DL. - 13 400|
|Biosolids, sludge (ng/g dw)b||U.S.A., Denmark, Faroe Islands, Finland, Iceland, Norway, Sweden, Spain||2002-2010||0.31 - less than 200|
|Wastewater, effluent, storm water (ng/L)||Sweden||2009-2010||less than DL - 1.2|
|Biota - aquatic (ng/g lw)||Canada, Hendrickson Island, Germany, Faroe Islands, Spain, Iceland, Norway, China, South Korea, Japan, Brazil,||1979-2011||less than DL - 93 000|
|Biota - terrestrial and avian (ng/g lw)||Canada, U.S.A., Chafarinas Islands, Spain, Finland, Sweden, Norway, Czech Republic, Iceland, Germany, Italy, China, Indonesia, South Korea, Tasmania, South Africa||2000-2010||less than DL - 18 000|
Abbreviations: DL = detection limit.
a See supporting documentation (Environment and Climate Change Canada 2015) for references and study details.
b Although wastewater system effluent and sludge/biosolids are not "environment", they represent a direct source to the environment and are included in this table.
8. Environmental Fate and Behaviour
8.1 Environmental Distribution
DP is expected to be released primarily from industrial sources to wastewater (a pathway to surface water and the soil environment) and may undergo some migration from consumer or commercial products to the atmosphere as a non-reactive flame retardant with potential for some release from polymers (CECBP 2008). DP is very likely highly removed by adsorption to sludges in wastewater treatment plants and can be applied to agricultural soils during biosolid amendment. Level III fugacity modelling (Table 8-1) using the updated EQC model (v1.0, 2012), was applied to describe the fate for these expected modes of entry into the environment. Generally, the results of Level III fugacity modelling show that DP is expected to predominantly reside in soil and/or sediment, depending on the compartment of release.
|Substance released to:||Air (%)||Water (%)||Soil (%)||Sediment (%)|
Very low water solubility (2.85 x 10-7 mg/L), low vapour pressure (6.57 x 10-11 Pa at 25°C) and very high partition coefficients (log Kow of 8.78, estimated log Koc of 6.65) suggest that DP released into the environment will be less likely to partition into and/or remain in air and water, moving instead to the sediments and soil. If released to air, a small fraction (less than 1%) of DP is expected to remain in air (in gas phase), with most of the substance depositing to soil and water with further partitioning to sediment. However, based on predicted patterns of transport (see description below), the small mass of DP that remains in air has the potential for dispersion.
The high partition coefficients indicate that DP released into surface water from wastewater is expected to adsorb to the organic fraction of suspended solids and sediments, with less than 4% remaining in water. However, as in the case with air, the small fraction remaining is likely to remain in water and has the potential for some transport (e.g., particle transport). Based on its high log Koc, once in the sediment, DP is not expected to be mobile and may remain in this compartment with little degradation.
When DP is released to soil (i.e., through biosolid application to agricultural lands), the majority of the mass fraction is expected to become adsorbed to soil (99.9%) based on its high estimated log Koc and hydrophobic nature. Based on its low vapour pressure, DP is not expected to evaporate (volatilize) from dry soil surfaces, and is therefore likely to remain in soil. In addition, low degradation is expected in soil; therefore, DP is likely to remain in this compartment, with loss processes driven by soil burial or surface runoff. The results of Level III fugacity modelling (Table 8-1) support the expectation that DP predominantly resides in soil or sediment, depending on the compartment of release (New EQC 2011).
8.1.1 Long-Range Transport Potential
Predicted log Koa (12.99) and log Kaw(-4.22 - -3.52) values for DP suggest low potential to reach the Arctic (Wania 2006, Brown and Wania 2008). The substance is identified as highly sorptive, sorbing to particles in atmospheric and aqueous media, and therefore, particle settling is predicted to limit long-range transport (Brown and Wania 2008). However, if particle-bound transport is more efficient than expected, it is possible that DP could be transported to remote regions, such as the Arctic.
Xiao et al. (2012) detected DP in 11 out of 14 high-volume air samples taken at a remote station in Alert in Nunavut, Canada (less than 0.05 pg/m3 to 2.1 pg/m3), and DP detection was primarily associated with particles. DP was detected in all air samples in Canada's western sub-Arctic (Little Fox Lake, Yukon Terrirory) between August 2011 to December 2014 (total DP from 0.1 to 1.8 pg/m3), under the Canadian Northern Contaminants Program (Yu et al. 2015). DP has been detected in wolves from remote Canadian Arctic locations (Muir et al. 2014), but was not detected in Arctic Beluga whales (Shen et al. 2012).
Xiao et al. (2012) also took monthly integrated samples at a remote station close to Nam Co Lake on the Tibetan Plateau, from 2006-2008. No DP was detected at this remote location. When compared to levels reported for low altitude sites, these results suggest orographic precipitation (relief rainfall) may limit the transport of DP to higher elevations. Although DP production is limited to China and North America, many studies have measured DP elsewhere. European studies report air concentrations ranging from 0.58 pg/m3 in Rao Sweden to 9.4 pg/m3 in Lille Valby, Denmark (TemaNord 2011). DP was detected in all particle phase atmospheric samples collected from Svalbard in the European Arctic in 2012 through 2013, ranging from 0.05 to 5 pg/m3 (Salmova et al. 2014). A study performed by Möller et al. (2010) detected DP in air and seawater from remote locations from the Arctic East Greenland Sea to Antarctica. For example, the study measured concentrations of 0.05-4.2 pg/m3 in 2-6 day samples of marine boundary layer air over the Atlantic Ocean. Furthermore, samples collected by the Global Atmospheric Passive Sampler (GAPS) have also reported relatively high levels in remote locations (Sverko et al. 2011). This latter study extends across all seven continents and has reported air concentrations ranging from below detection limits up to greater than 75 pg/m3 in Cape Grim, Tasmania (Moller et al. 2012, Sverko et al. 2011, Xian et al. 2011). Recent studies in remote Norwegian high Arctic locations have measured DP in air, seawater, fjord sediment, soil, moss, dung (reindeer and bird), eggs, as well as bird, seal, and polar bear tissue (Ma et al. 2015, Na et al. 2015, Vorkamp et al. 2015). These empirical studies suggest DP is available for long-range transport.
The OECD POPs Screening Model can be used to help identify chemicals with high persistence and long-range transport potential (OECD 2006). The Characteristic Travel Distance (CTD) calculated for DP using the OECD model is 2508 km, indicating that DP has potential for transport in air; however, this is below the boundary (5097 km, CTD of PCB-28) suggested for global pollutants by Klasmeier et al. (2006). The model also calculates an overall persistence (Pov) of 260 days, as well as the transfer efficiency (TE), which is the percentage of emission flux to air that is deposited to the surface (water and soil) in a remote region. The TE for DP was calculated to be 9.7%, which is above the boundary of 2.248% (PCB-28) established based on the model's reference substances empirically known to be deposited from air to soil or water. The high TE means that DP could be deposited to some degree to Earth's surface in remote regions.
In general, while DP (based on physical chemical properties and some models) would not be expected to be a high concern for long-range transport in the gas phase, based on a high predicted transfer efficiency and detection of DP in remote areas, the role of particle-bound transport requires further consideration.
8.2 Environmental Persistence
Based on likely releases and predicted partitioning characteristics of DP, and considering the measured environmental concentrations, environmental persistence will be considered in all media compartments. In order to evaluate the weight of evidence for persistence of DP, empirical and modelled data are considered. Relevant transformation processes for DP include photodegradation, and biodegradation. Empirical data from industry degradation studies described in this assessment are taken from public industry submissions to other government agencies (e.g., IUCLID, US EPA), as the original industry studies were not available to the Government of Canada. Therefore, empirical data from industry studies could not be reviewed for validity.
Generally, model predictions support experimental findings that aerobic and anaerobic biodegradation of DP is very limited and that DP is expected to be persistent in water, soil, and sediment. Modelled predictions for DP persistence in air are not consistent, and suggest a half-life less than 0.5 day for photolysis and a half-life of 160 days for ozone reaction. However DP sorption to airborne particles is expected, which would lower the photolysis rate and result in a longer half-life in air. An overall persistence (Pov) of 260 days is predicted by the OECD POPs tool.
8.2.1 Abiotic Degradation
No empirical degradation data were found for DP in air. The predicted half-life for atmospheric degradation of DP due to reaction with the hydroxyl radical is 0.468 days (12-hr day, AOPWIN 2010). The results of AEROWIN (2010) predict a high fraction of DP sorption to airborne particles (Phi = 0.9 to 0.96), and therefore, that the rate of DP photodegradation is likely lower than predicted (i.e., half-life longer than predicted 0.468 days). Sverko et al. (2011) suggest that air modelling of DP (based on gas phase) underestimates the half-life due to its association with particles which would slow reaction rates. An overall persistence (Pov) of 213 days is predicted by the OECD POPs tool for DP emission to air, with the model estimating 98.82% of substance in air being partitioned to aerosols. An overall persistence (Pov) for all compartments (air, water, soil) is predicted at 260 days.
The most recent IUCLID data set for DP (US EPA 2009) summarizes a 1979 study reporting limited photodegradation (less than 10%) of DP in water (eutrophic lake water and distilled water) irradiated under a mercury borosilcate lamp for 168 hours (photolysis half-life was estimated at greater than 24 years). The light source did not replicate natural sunlight but provided several lines of high photon fluxes in the solar spectral region (greater than 290 nm) which were reported to increase the rate of phototransformation over that expected from natural sunlight.
Sverko et al. (2008) initiated a simple photodegradation study which irradiated a 100 ng/mL isooctane solution of each DP isomer to UV light (λ ~ 365 nm) for a 30-day period. A decrease in parent DP concentration of 10% at 168 hour (h) and a further loss of 40% at 264 h and 65% at 504 h were observed. Anti-DP appeared to degrade more readily than the syn-DP stereoisomer. Similarly, Wang et al. (2011) conducted three photolytic experiments and found dechlorination (DP [-Cl+H] and [-2Cl+2H] degradation products), but also other unidentified DP-degradation products.
DP does not contain functional groups expected to undergo hydrolysis.
|Medium||Fate process||Degradation value||Degradation endpoint / units||Methods||Reference|
|Air||Atmospheric oxidation||0.468 daysb||half-life/days||Model||AOPWIN 2010a|
|Air||Ozone reaction||160.12 days||half-life/days||Model||AOPWIN 2010a|
|Water||Photolysis||greater than 24 years|
(less than 10%, 168 hours)
|Mercury lamp with borosilicate immersion well (solubilizer used)||Chou et al. 1979|
|Isooctane (Solvent)||Photolysis||10% at 168 h 40% at 264 h 65% 504 h||% degradation/hour||UV light (λ greater than 365 nm)||Sverko et al. 2008|
a EPIsuite (2010-2012).
b AEROWIN (2010) predicts high fraction of DP absorption to airborne particles (Phi =0.9 to 0.96); therefore the rate of DP photolysis is likely lower than predicted (i.e., half-life longer than predicted).
c Model does not provide an estimate for this type of structure.
Laboratory tests have shown DP is not likely to biodegrade under aerobic conditions. An activated sludge biodegradation test (modified MITI OECD 301C) reported 0.6% biodegradation in 2 weeks (US EPA 2011), and a 21-day test using sewage sludge found 0% biodegradation (US EPA 2009).
The four ultimate biodegradation (BIOWIN (2010) submodels, as well as Catalogic (2012) and TOPKAT (2004), show that biodegradation is very slow or recalcitrant and that the half-life in water would be on the order of several months. In addition, a primary biodegradation model, BIOWIN Sub-model 4 (primary survey model), predicts the substance is recalcitrant. This is comparable to the overall persistence (Pov) of 260 days that is predicted by the OECD POPs tool.
DP appears to be well covered by the number of fragments and molecular size based on the domain of applicability for BIOWIN submodels 5 and 6 (aerobic biodegradation, MITI). The molecular weight falls within the range covered by BIOWIN Submodels 3 and 4 (aerobic biodegradation, Expert Survey), however the domain includes substances with fewer aliphatic chloride fragments and fewer fragments containing carbon with four single bonds and no hydrogens. Although this introduces further uncertainty regarding the modelled results from submodels 3 and 4, the degradation predictions are in agreement with other modeled data as well as results from the empirical studies. They are also consistent with DP's complex structure that is not amenable to microorganism attack.
Biodegradation modelling by both TOPKAT and Catalogic (2012) suggests that DP biodegrades slowly. TOPKAT suggests that probability of aerobic biodegradability for DP is nil (within the domain of the training sets). Catalogic (2012) identifies three low probability products (probability to obtain is 1 to 2 %, representing 7% quantity each, relative to parent DP) (C18H13Cl11O1, C18H12Cl10O1, C18H12Cl10O2) that differ from dechlorination products identified in photodegradation studies described above.
The existing data for anaerobic degradation of DP suggests that if the substance degrades, it does very slowly. Data from a 1979 industry study indicated 0% anaerobic biodegradation over 2 to 6 weeks by sewage sludge microorganisms, and no metabolites were identified (European Commission 2008, US EPA 2011).
These aerobic and anaerobic biodegradation tests, as well as modelling results, indicate that the half-life in water is likely to be longer than several months and that the substance is therefore likely to persist in water (Table 8-3). Using an extrapolation ratio of 1:1:4 for a water: soil: sediment biodegradation half-life (Boethling et al. 1995), the half-life in soil is also longer than several months and the half-life in sediments is greater than a year, indicating that DP is expected to be persistent in soil and sediment.
|Medium||Fate process||Degradation value||Degradation endpoint / units||Methods||Reference|
|Activated sludge||Bio-degradation||0.6%||2 weeks|
|OECD 301C (Modified MITI test)||US EPA 2011|
|Water sewage sludge||Aerobic Bio-degradation||0%||21-day Biodegradation/%||Standard methods for examination of water and wastewater (13th ed) 1971||US EPA 2009; US EPA 2011|
|Anaerobic sewage sludge||Anaerobic Biodegradation||0%||2 and 6 weeks biodegradation/%||Radiolabeled DP in effluent with anaerobic sewage sludge organisms||US EPA 2009; US EPA 2011|
|Water||Primary Bio-degradation (aerobic)||0.7766a|
|greater than several months||QSAR Model||BIOWIN 2010d|
|greater than several months||QSAR Model||BIOWIN 2010e|
|greater than several months||QSAR Model||BIOWIN 2010f|
|greater than several months||QSAR Model||BIOWIN 2010g|
|Water||Bio-degradation (aerobic)||% BODc = 1(BOD = 1 in training set)|
|greater than several months||QSAR Model||Catalogic 2012|
|greater than several months||QSAR Model||TOPKAT 2004|
a Output is a numerical score from 0 to 5.
b Output is a probability score.
c BOD – Biological Oxygen Demand.
d Sub-model 4: Expert Survey (qualitative results).
e Sub-model 3: Expert Survey (qualitative results).
f Sub-model 5: MITI linear probability.
g Sub-model 6: MITI non-linear probability.
8.3 Potential for Bioaccumulation
The evaluation of DP bioaccumulation potential examines several parameters, including physical chemical properties, bioconcentration factor (BCF), biomagnification factor (BMF), trophic magnification factor (TMF), and bioaccumulation factor (BAF). The role of metabolic biotransformation in determining bioaccumulation potential is also discussed. Empirical and some modelled data were considered.. Most original (unpublished) industry experimental bioaccumulation/bioconcentration studies are not available to the Government of Canada, and data are only available from secondary sources (i.e., IUCLID format), therefore limiting the evaluation of study reliability and details. Bioacccumulation potential related data are considered using a weight of evidence approach.
Based on its physical and chemical properties (e.g., moderately large maximum diameter, very low water solubility, high log Kow, and low experimental BCF), DP is expected to have a low bioconcentration potential. However, monitoring studies from many parts of the world have reported measurable DP in aquatic and terrestrial organisms. Data for field-based BMF, BAF, and BSAF support that DP bioaccumulation and biomagnification occur. Studies of metabolism in wildlife (fish and birds) show no evidence of metabolic transformation products, suggesting little to no metabolism of DP. The log Kow for DP (8.78) is considered outside of the model domain (8.2) for the mass-balance three trophic level BCFBAF model (Arnot and Gobas 2003) and the (Q)SAR-based model (Dimitrov et al. 2005). Although modelling BCF and BAF for DP is undertaken, it is recognized that the predictions are extrapolated beyond empirical data within the model, and are thus less certain and considered a supporting line of evidence and included with supporting documentation (Environment and Climate Change Canada 2015).
8.3.1 Bioconcentration Factor (BCF)
Experimental BCF data for DP exist from a few older studies (e.g., Boudreau 1973, Gara and Rauisina 1975, Chou et al. 1979, Zitko 1980, CHIRP c2008); see Environment and Climate Change Canada (2015). However, none are considered reliable due to various limitations (e.g., reported exposure concentrations greatly exceeding water solubility, short exposure times, and use of dispersants). The CHRIP study (c2008) exposed Japanese carp to DP for 8 weeks (0.0027 mg/L and 0.000027 mg/L, i.e., 2 to 4 orders of magnitude greater than water solubility), resulting in a BCF ranging from 14 to 121. Zitko (1980) found no DP uptake from water to fish tissue during 96 hours, but DP accumulation from food to tissue was observed (see next section). These studies do, however, indicate that uptake of DP in fish occurs, with DP concentrations reaching up to 8.8 mg/kg ww after 30 days in one study (Boudreau 1973). Furthermore, given the very low solubility of DP, it is expected that steady state (and therefore maximum DP tissue concentrations) would not be reached for a very long time (BCF could therefore be underestimated). For example, if assuming exceeding water solubility is the only limitation of the BCF studies, then recalculation of the BCF with a correction to reported water concentrations as described in Arnot and Gobas (2006), could result in much larger BCF values.
Due to the limitations in the available DP bioconcentration studies, kinetic mass-balance modelling was conducted (see Supporting Documentation, Environment and Climate Change Canada 2015). However, the log Kow for DP (8.78) is considered outside of the model domain (approximately 8.2) for the mass-balance three trophic level BCFBAF model (Arnot and Gobas 2003) and the (Q)SAR-based model (Dimitrov et al. 2005). Although modelling BCF for DP is undertaken, it is recognized that the predictions are extrapolated beyond empirical data within the model, and are thus less certain. Despite this limitation, results are generally consistent with observed empirical data, suggesting that bioconcentration is insignificant. The detailed results of the BCF modelling are given in the supporting documentation (Environment and Climate Change Canada 2015).
Recent investigations relating fish BCF data and molecular size parameters (Dimitrov et al. 2005, Sakuratani et al. 2008) suggest that the probability of a molecule crossing cell membranes as a result of passive diffusion declines significantly with increasing maximum diameter (Dmax). Based on the BCFmaxModel with Mitigating Factors (Dimitrov et al. 2005), the maximum diameter of DP ranges from 1.35 to 1.48 nm. This suggests that the uptake rate of DP could be restricted to some degree by from steric effects at the gill surface.
At a log Kow of 8.78, the predicted bioavailable fraction of DP in the water column (excluding loss from volatilization) according to mass-balance fish models is 0.005%, which suggests that uptake from water via the gills is not a dominant exposure pathway for DP. It also suggests that the dietary uptake of DP contributes a significant proportion to the overall uptake of this chemical when both water and dietary considerations are considered (i.e., bioaccumulation).
8.3.2 Bioaccumulation Factor (BAF)
BAF studies for DP are limited. A study conducted in a natural reservoir in South China near e-waste recycling plants compared DP in water and biota, and reported syn-DP and anti-DP were both significantly biomagnified in freshwater organisms (Wu et al. 2010). Data from DP in water (dissolved) and tissue (wet weight for 2 invertebrates, 4 fish, and 1 reptile) collected in 2006 are used for calculating BAF values. DP BAF values ranged from 135 to 25 118, with 4 of 6 test species showing significant/high bioaccumulation (authors identify this as log BAF greater than 3.7). There were some uncertainties associated with this study including limited details on the analytical methodology, small sample sizes, and no information about water/biota collection times to support assumptions of "steady state". Nevertheless, DP was detected in all aquatic species with concentrations from 19.1 to 9630 ng/g lipid wt.
Zitko (1980) observed DP accumulation from food to tissue over a 42-day feeding period: a corrected accumulation factor of 0.024 was reported for the concentration at 28 days. However, there are several limitations with the methods used in this study for a hydrophobic substance like DP (e.g., Sverko et al. 2011). While the results are not reliable, the study does demonstrate uptake of DP in fish (maximum reported tissue concentration = 176 μg/kg ww after 15 days).
Although few BAF values have been presented for Canadian aquatic systems, a preliminary examination of reported DP water concentrations (4.89 to 14 pg/L) and DP in fish tissue (70 to 1600 pg/g ww) from Lake Ontario collected within the last number of years suggests that high bioaccumulation in fish could be occurring (see data from Reiner et al. 2006; Tomy et al. 2007; Muir et al. 2011; Ismail et al. 2009; Shen et al. 2010; Shen et al. 2011a; Venier et al. 2014). Muir et al. (2014) reported a DP logBAF for Lake Ontario zooplankton of 9.1 (BAF=1.26 x 109), and for mysids of 8.6 (BAF=3.98 x 108), which suggest very high bioaccumulation.
Due to the lack of empirically derived BAF data available in the literature, metabolism corrected kinetic mass-balance modelling was conducted to help fill this data gap; see Environment and Climate Change Canada (2015). However, at a log Kow of 8.78, the model is predicting bioaccumulation for a higher Kowthan the substances with measured BAFs (e.g., PCBs) contained in the model's dataset, and therefore results are less certain. Despite this limitation, results are generally consistent with observed empirical BAF data (Wu et al. 2010) suggesting bioaccumulation potential is high.
Studies reporting Biota-Sediment Accumulation Factors (BSAF) greater than 1 are indicative of bioaccumulation in biota from sediment. Studies were identified with reported BSAF values ranging from 0.0003 to 2.8, with the majority reported as less than 1 (Table 8-4). However given that fish (rather than a sediment dwelling organisms) were often used in the reported BSAF studies, it is not surprising that BSAFs were found to be less than 1, as sediment is not the only (or primary) route of DP exposure to these organisms).
Although limited, the BAF data show potential for high DP bioaccumulation. In general, these studies support the BMF and TMF studies presented in section 8.3.3 to suggest bioaccumulation of DP does occur.
|Method||Test organism||Duration||BAF or BSAF (L/kg)||Reference|
|BAF-field samples||Lake Ontario, Canada||NA|
|3.98 x 108|
|Muir et al. 2014|
|BAF-field samples||Lake Ontario, Canada||NA|
|1.26 x 109 (Zooplankton)||Muir et al. 2014|
|BAF-field samples||South China reservoir|
|135 (Chinese Mysterysnail)||Wu et al. 2010|
|BAF-field samples||South China reservoir|
|less than 5000 (Northern Snakehead)||Wu et al. 2010|
|BAF-field samples||South China reservoir|
|greater than 5000 (Prawn)||Wu et al. 2010|
|BAF-field samples||South China reservoir|
|greater than 5000 (Crucian carp)||Wu et al. 2010|
|BAF-field samples||South China reservoir|
|greater than 5000 Mud carp||Wu et al. 2010|
|BAF-field samples||South China reservoir|
|25 118 (Water Snake)||Wu et al. 2010|
|Wang et al. 2012|
|BSAF-field samples||Crucian Carp (Carassius auratus)||NA||Total-DP: 0.004|
|Zhang et al. 2011b|
|BSAF-field samples||Mud Carp (Cirrhinus molitorella)||field samples||Total-DP: 0.025|
|Zhang et al. 2011b|
|BSAF-field samples||Northern Snakehead (Ophicephalus argus)||NA|
|Zhang et al. 2011b|
|BSAF -field samples||Lake Trout||NA|
|Shen et al. 2014|
Abbreviations: NA = not available
8.3.3 Biomagnification Factor (BMF)
A BMF exceeding 1 indicates that biomagnification is potentially occurring, and may be considered an indicator of the potential for uptake and accumulation in biota. Table 8-5 presents empirical BMF data for DP.
Recent Canadian Arctic research in remote areas (western Nunavut, Bathurst region) analyzed total body burden concentration for caribou (Rangifer tarandus groenlandicus) and wolf (Canis lupus) and found some terrestrial BMF values for anti-DP greater than 1 (e.g., 3.4); however, none were statistically significant (Muir et al. 2014). A BMF for syn-DP was not reported.
Yu et al. (2013) examined biomagnification of DP within terrestrial avian food chains in Beijing, China. BMF values (lipid-normalized) were determined for predator owls (Bubo bubo and Athene noctuaa) and prey (Norway Rat, Rattus norvegicus), as well as for common kestrel (Falco tinnunculus) and its prey, the Eurasian tree sparrow (Passer montanus). BMF values were greater than 1 for the owl-rat food chain, but less than 1 for the sparrow-kestral foodchain. No stereoselective bioaccumulation was found for DP isomers in the investigated species.
A field study in South China (She et al. 2013) examined a small herbivorous food chain (paddy soils to rice plant to apple snails) and found that lipid normalized DP BMFs for rice plant (Oryza sativa) to apple snail (Pomacea canaliculata) ranged from 0.59 to 7.9, with mean values of syn-DP: 3.1, and anti-DP: 2.3 (Table 8-5). These BMFs were comparable to those determined for the PBDEs in the same samples.
Although BMFs of 5.2 for syn-DP and 1.9 for anti-DP were reported in a laboratory study on juvenile rainbow trout exposed to DP via dietary uptake (Tomy et al. 2008), a recent evaluation of the study (Arnot and Quinn, unpublished manuscript) suggests a ~100 fold error in the calculation, and estimates that lipid normalized BMFs are actually ~ 0.089 (syn-DP) and 0.046 (anti-DP). In the original study, sixty fish were exposed for 49 days, followed by a depuration phase of 112 days. The Tomy et al. (2008) study suggests syn-DP as more bioavailable (or more slowly transformed) than anti-DP (Table 8-5). The authors speculate that structural conformation differences of the pendant chlorocyclopentene moieties of the anti-isomer make it more susceptible to biological attack. Screening of fish liver suggests that, if DP metabolites are detected in aquatic food webs, it is likely not due to in vivo biotransformation of the parent compound. Despite the purposely high DP dose, no dechlorinated, hydroxylated, methoxylated, or methyl sulfone DP degrades were detected in liver extracts.
The extent of bioaccumulation of the syn- and anti-isomers of DP was assessed in archived food web samples from Lake Winnipeg and Lake Ontario (Tomy et al. 2007). Biomagnification was assessed using both calculated trophic level adjusted biomagnification factors (BMFTL) for the predator-prey relationships, as well as by trophic magnification factors (TMFs) (see section 8.3.4). For Lake Winnipeg, biomagnification was only found for the walleye-whitefish feeding relationship for the anti-DP isomer (BMFTL greater than 11). The authors suggest that this indicated a stereoselective elimination of the syn-isomer in preference to the anti-isomer by walleye or that walleye can metabolize the syn-isomer more readily. In Lake Ontario, the trout-smelt feeding relationship showed BMFTL values greater than 1 (anti-DP:11, syn-DP:12), and trout-alewife BMF was equal or just below 1. The authors suggest that lake trout, unlike walleye, are not stereoselectively accumulating or metabolizing the isomers, supporting their hypothesis of interspecies differences in bioaccumulation and biotransformation.
|Test organism||BMF (/kg)||Dietary Assimilation Efficiency (α; %)a||Reference|
|wolf/caribou||anti-DP: 3.4 ± 2.1|
(BMF greater than 1)
|NR||Muir et al. 2014|
|kestrel/sparrow||(BMF less than 1)|
Total TP: 0.32
|NR||Yu et al. 2013|
|owl/rat||(BMF greater than 1)|
Total TP: 2.0
|NR||Yu et al. 2013|
|apple snails/rice plant||(BMF greater than 1)|
syn-DP: 3.1 (0.63 to 7.9)
anti-DP: 2.3 (0.59 to 4.7)
|NR||She et al. 2013|
|(BMF less than 1)|
~0.046 (anti-DP) to
|3.9 (anti-DP) to 6.0 (syn-DP)||Arnot and Quinn (unpublished manuscript); Tomy et al. 2008|
whitefish (Lake Winnipeg)
|NR||Tomy et al. 2007|
|walleye/ whitesucker (Lake Winnipeg)||syn-DP: 0.6||NR||Tomy et al. 2007|
|walleye/goldeye (Lake Winnipeg)||anti-DP: 0.8|
|NR||Tomy et al. 2007|
|goldeye/ zooplankton (Lake Winnipeg)||syn-DP: less than 0.1||NR||Tomy et al. 2007|
|Lake trout/alewife (Lake Ontario)||anti-DP: 0.9|
|NR||Tomy et al. 2007|
|Lake trout/smelt (Lake Ontario)||anti-DP: 11|
|NR||Tomy et al. 2007|
|Lake trout/sculpin (Lake Ontario)||anti-DP: 0.1|
|NR||Tomy et al. 2007|
|sculpin/diporeia (Lake Ontario)||anti-DP: 0.2|
|NR||Tomy et al. 2007|
Abbreviation: NR = Not reported.
a This is also called the absorption efficiency (ED or α) and is a measure of the transfer of a chemical from the gastrointestinal tract (GIT) into the organism relative to the total amount of chemical the organism is exposed to from the diet.
Although there are some uncertainties in the available biomagnification data, it is reasonable to consider that DP would biomagnify in food chains given its physical and chemical properties (high log Kow of 8.78 and log Koaof 12.99). DP may have the potential to biomagnify in terrestrial food webs as suggested by Gobas et al. (2003) and Kelly et al. (2007). However, these partition coefficients do not account for physiological parameters such as metabolism, and the available empirical data on bioaccumulation. The available biomagnification data suggest that BMFs for DP can exceed 1 in some feeding relationships, which suggests that dietary exposures may significantly contribute to trophic transfer and food web accumulation in the environment.
In a study of dietary efficiency of chemicals by fish, Xiao et al. (2013) examined 15 chemicals, including DP, for gross absorption efficiency (Ed) using a benchmarking method (single exposure). Study fish were fed a single meal of contaminated feed, and then analyzed for chemical distribution after 5 days. DP apparent and "benchmarked" absorption efficiency (Ed) were estimated at 0.37 and 0.28, respectively.
8.3.4 Trophic Magnification Factor (TMF)
The TMF is a measure of the average biomagnification potential of a substance within a studied food web under field conditions, and is estimated by correlating the normalized substance concentrations in biota against different trophic levels.
Syn-DP and anti-DP were both significantly biomagnified in a food web of a reservoir nearby electronic waste recycling workshops in South China (Wu et al. 2010; see BAF section for study details). The study, conducted in 2006, determined TMFs of 11.3 (syn-DP) and 6.6 (anti-DP), indicating that both isomers of DP were significantly biomagnified throughout the food web. The TMF of syn-DP was almost two times greater than that of anti-DP, suggesting greater syn-DP biomagnification potential than that of anti-DP in the present food web. The depletion of anti-DP isomers in organisms compared to abiotic samples and a decrease in presence up the trophic levels also suggests possible stereospecific metabolism of the anti-DP and selective uptake of syn-DP.
Anti-DP and total DP were significantly biomagnified in an aquatic foodweb (two invertebrate and five fish species) from the Beijing-Hangzhou canal, located near the DP manufacturing facility in the Jiansu province of China (Wang et al. 2015). TMF values (lipid normalized) of 3.1 (syn-DP), 1.9 (anti-DP), and 2.2 (total DP) were reported, however the TMF vaue for syn-DP was not significant. Similar findings were reported in a study of five fish species from 22 rivers across South Korea in 2008 (Kang et al. 2010). The proportion of anti-DP found in samples from urban-industrial sites was significantly lower than the technical DP standard, demonstrating that DP isomers exhibit varying bioaccumulation behaviours, with syn-DP bioaccumulating in biota more than anti-DP.
The lipid normalized TMFs for DP were determined based on archived food web samples from Lake Winnipeg and Lake Ontario (Tomy et al. 2007). While there were differences in the TMFs of the isomers in the Lake Winnipeg food web, no statistically significant TMFs for either isomer were found for the Lake Ontario food web. A TMF of 2.5 for anti-DP and a TMF less than 1 for syn-DP were found in Lake Winnipeg.
8.3.5 Other Bioaccumulation-related Studies
A recent study of DP used liver microsomes of Montreal-breeding ring billed gulls (Larus delawarensis) to examine in vitro metabolism of DP (Chabot-Giguere et al. 2013). No degradation of either isomer occurred over the 90 minute assay.
In a study of European Starling (Sturnus vulgaris) eggs, DP was one of the most prevalent flame retardants detected in sampling sites across Canada (along with certain PBDEs). DP (both isomers) was detected in 41% of the examined egg pool homogenates with concentrations up to 24 ng/g ww (Chen et al. 2013), indicating maternal transfer.
A study of DP tissue distribution in two bottom fish species (northern snakehead and mud carp) in South China determined preferential distribution to liver to muscle for syn-DP, and a high persistent retention in the brain compared to liver for anti-DP, suggesting the latter isomer can cross the blood-brain barrier in fish (Zhang et al. 2011b). Median DP levels ranged from 0.18 to 39.1 and 0.22 to 52.9 ng/g ww for synand anti-DP, respectively.
These wildlife studies provide variable evidence suggesting bioavailability and metabolism of DP, possibly suggesting that bioavailability and metabolic potential for DP may be species-specific and may vary between aquatic and terrestrial organisms.
8.4 Summary of Environmental Fate
DP is expected to be released to the environment through wastewater. There may be a potential for migration of DP from plastics to the atmosphere given that the substance is added to the polymer matrix and thus could leach to some extent, but there is currently no monitoring evidence to confirm its significance. A strong tendency to sorb to the solid phase in various media (including suspended air particles) indicates that DP will reside in biosludges, sediments, suspended air particles, and will be transferred to soil from dry deposition and application of biosolids to agricultural lands. Exposure to organisms in water is expected to be low. DP's high intrinsic persistence suggests that long-term exposures can be expected in sediment and soil with a potential for significant build-up in near-field environments from continuous emissions. The removal process from the environment would include sediment and soil burial. DP might be expected to undergo long-range transport in air and deposition to remote environments due to fine particle transport as has been evidenced with other hydrophobic flame retardants with high air particle sorption. Given this substance's bioavailability, long-term exposure may result in elevated tissue levels in biota, food web transfer, and biomagnification.
9. Potential to Cause Ecological Harm
9.1 Ecological Effects Assessment
Empirical data for DP, as well as relevant comparative data for the structural analogues, chlordane and mirex, were considered in the weight-of-evidence for assessing the ecological effects of DP. DP (Q)SAR toxicity modelling generally indicated no effects at saturation. The log Kow for DP also exceeded model cut-offs, and the substance was poorly covered by the domains of the toxicity models. Therefore, modelled predictions are considered uncertain.
For DP, results from the available empirical aquatic toxicity studies have high uncertainty and questionable applicability, mainly because treatment concentrations exceeded the DP water solubility limit by orders of magnitude. Furthermore, given that DP preferentially partitions to soil or sediment compartments, dissolved phase aquatic toxicity is not the most environmentally relevant form of testing for the effects of this substance. For this reason, potential aquatic effects are evaluated considering the critical body residue (CBR) approach and less weight is given to water-phase exposure in the evaluation of ecological effects of DP.
DP is classified as base surface narcotic/neutral organic and/or vinyl/allyl halide for aquatic toxicity by ECOSAR. DP has limited results available within the OECD QSAR Toolbox (2012) profile; OECD protein binding is designated as high (Class 3) for DP. As well, protein binding is identified as greater than 100 days (OASIS v.1.2, OECD). While DP's mode of action may not be completely understood for aquatic organisms (e.g., sediment organisms), its analogues, the cyclodiene insecticides (e.g., chlordane), are generally considered neurotoxicants for terrestrial organisms (EC 1999).
Based on the results of sediment and soil chronic toxicity testing, using the analogues chlordane and mirex, DP has the potential to cause effects at low concentrations to soil organisms such as earthworms and insects. DP, based on its analogue, chlordane, also has potential to cause effects at low concentrations to sediment organisms. DP does not show potential for effects on wildlife based on current rodent and avian studies.
184.108.40.206 Aquatic Effects from Dietary Exposure
The toxicity potential from dietary uptake was investigated based on the predicted behaviour of DP, i.e., a high degree of partitioning to sediment and soil coupled with a high degree of environmental stability and bioaccumulation potential via the diet. The critical body residues (CBR) concept was applied to investigate the potential for adverse effects in a 5% lipid fish from the dietary uptake of DP. This concept considers whether the uptake of a chemical from the environment can accumulate to critical body burden levels associated with effects like mortality as a result of baseline narcosis. McCarty and Mackay (1993) and McCarty et al. (2013) have shown that internal concentrations of neutral narcotic chemicals in fish causing death are fairly constant at about 2-8 mmol/kg for acute exposures and 0.2-0.8 mmol/kg for chronic exposures. McCarty and Mackay 1993 provide the mathematical formula to estimate critical body residue in fish as follows:
CBR = BAF (5% lipid) x water concentration of chemical / MW,
- CBR =
- critical body residue in fish (mmol/kg)
- BAF (5% lipid) =
- can be BAF or BCF lipid normalized to 5% (L/kg)
- MW =
- molecular weight of the substance (g/mol) Chemical concentration in water (mmol/L)
Given the lack of available BAF data for fish in the literature, the CBR was calculated using the modelled BAF value of 2.12 x106. The water solubility was used as the environmental concentration in surface water (i.e., if environmental concentrations in surface waters approach levels equivalent to the water solubility limit, then it can be ascertained whether there is a potential for adverse effects through the food web; e.g., fish and mammalian piscivores) (see Table 9-1).
See Environment and Climate Change Canada (2015) for assumptions regarding the CBR approach. As a second approach, the highest DP concentration reported in fish tissue from Canada (0.85 ng/g ww) was converted to CBR units and compared to the threshold. In addition, as DP exposure via diet is the most likely exposure pathway, the biota-diet fugacity was also determined; see Environment and Climate Change Canada (2015).
|Approach||DP Water Concentration (mg/L)||BAF (L/Kg)||CBR (mmol/kg)||Meets Acute CBR effects for baseline narcosis (lethality)|
(2 - 8 mmol/kg)
|Meets Chronic CBR effects for baseline narcosis (lethality)|
(0.2 - 0.8 mmol/kg)
|Modelled BAF||2.85 x 10-7a||2.12 x 106b||9.24 x 10-4||no||no|
|Biota sample||-||-||1.30 x 10-6c||no||no|
a Water solubility limit used as DP water concentration.
b Highest DP modelled BCFBAF (2010).
c Highest mean DP in Canadian fish tissue (Lake Trout, Lake Ontario) reported as 0.85 ng/g ww in Ismail et al. 2009).
The estimated CBRs are below thresholds for acute and chronic lethality. Using maximum Canadian fish tissue concentration compared with CBR thresholds also demonstrates that acute and chronic adverse effects are not expected. However, the potential for sublethal effects remains.
9.1.2 Empirical Studies in Sediment
As no sediment toxicity data were found for DP, mirex and chlordane sediment toxicity data were reviewed for read-across to DP. For mirex, while several aquatic toxicity studies for fish and invertebrates (e.g., crustacea) have been undertaken, and have shown high toxicity (Environment and Climate Change Canada1977, IPCS 1984), no sediment-based toxicity studies with usable toxicity endpoints were found.
For chlordane, Canadian interim sediment quality guidelines (ISQGs) have been established based on field determined data sets and spiked toxicity testing (e.g., marine organisms) (CCME 1999). The ISQG report presents a freshwater ISQG of 4.5 μg/kg dw and a probable effect level (PEL) of 8.87 μg/kg dw, as well as a marine ISQG and PEL of 2.26 and 4.79 μg/kg dw, respectively. Field studies where benthic invertebrate effects were associated with chlordane in sediment include a Canadian study conducted in Toronto Harbour, where decreased species richness and decreased abundance of chironomids were associated with a mean sediment chlordane level of 10.5 μg/kg dw (although other organochlorine pesticides were also present) (CCME 1999; Jaagumagi 1988; Jaagumagi et al. 1989). Another study identified a decrease in bivalve density in response to chlordane additions to New Zealand mid-tide sand flats, where near surface chlordane concentrations reached ~ 7.5 μg/kg dry fines (Pridmore et al. 1991). Toxicity studies identified in the ISQG report include a New Zealand study of the marine bivalve, Macoma Liliana, exposed to chlordane in sediment bioassays (Roper and Hickey 1994). The study showed significant sublethal avoidance movement out of chlordane dosed sediment at 20 μg/kg dw (96 h), and acute effects, LC10 and LC50, at 111 and 238 μg/kg dw. The ISQG report also discusses a study from Canada; a 96h LC50 of 120 μg/kg dw for chlordane was reported for the marine shrimp (Crangon septemspinosa) in sediment tests (McLeese and Metcalfe 1980). Another Canadian study by McLeese et al. (1982) reported a 288h LC50 of less than 5.8 mg/kg dw for the marine sandworm (Nereis virens). More recent studies of chlordane effects on sediment organisms generally examine mixtures of contaminants rather than effects directly attributable to chlordane alone.
|Test Organism||Test Type||Endpoint||Sediment chlordane in|
(μg/kg dry weight (dw))c
|sediment toxicity: survivala||96h LC50||120||McLeese and Metcalfe 1980|
sediment toxicity: survivala
|288h LC50||less than 5.8||McLeese et al. 1982|
sediment toxicity: survivala
|10d LC50||238||Roper and Hickey 1994|
sediment toxicity: survivala
|10d LC10||111||Roper and Hickey 1994|
sediment toxicity: avoidancea
|96h avoidance||20||Roper and Hickey 1994|
|Freshwater sediment (benthic) organisms||field sediment and benthic invertebrate data surveyb||decrease in species richness and Chironomidaeabundance||0.0105||CCME 1999|
|Marine sediment (benthic) organisms||experimental field sediment and benthic invertebrate data studya||decrease in bivalve density||~7.5 dry fines (near surface)||Pridmore et al.1991|
|Database of freshwater sediment organisms||N/A||Freshwater|
|Database offreshwater sediment organisms||N/A||Freshwater|
|Database of marine sediment organisms||N/A||Marine|
|Database of marine sediment organisms||N/A||Marine|
Abreviations: LC = lethal concentration; ISQG = interim sediment quality guidelines; PEL = probable effect level
a Technical chlordane used in study.
b Sampling survey of benthic invertebrates and sediments in Lake Ontario (Toronto Harbour); therefore likely exposure to chemical mixture, not only chlordane.
c Spiked-sediment toxicity tests for chlordane report the onset of toxicity to benthic organisms at higher concentrations than those observed in field studies. This is likely a result of the shorter exposure times of these laboratory studies, as well as exposure to chlordane only as opposed to chemical mixtures containing chlordane (Environment and Climate Change Canada 1998).
A CTV of 0.120 mg/kg is selected for DP (based on chlordane) in sediment. This CTV is the lowest endpoint representative of a sediment organism (Crangon septemspinosa) found in Canada. An assessment factor of 10 is used, to adjust a short-term acute toxicity endpoint (96 h LC50) to a chronic no-effect level, as well as an additional uncertainty factor of 10 is used to address inter/intra species variation (only 3 species tested). The resulting CTV is 0.0012 mg/kg dw. When this value is adjusted from test organic carbon content (0.28%) to standard sediment organic carbon content (4%) (Webster et al. 2004), the PNEC for sediment organisms is determined to be 0.0171 mg/kg dw. As the test sediment contained 0.28% OC, the maximum solubility of DP (based on analogue chlordane) in sediment was ~9.45 mg/kg dw; see Environment and Climate Change Canada (2015). The sediment solubility limit was therefore not exceeded under the conditions of the study.
9.1.3 Empirical Studies in Soil
As only one soil toxicity study for DP has been found, data for the analogues chlordane and mirex were also reviewed.
Zhang et al. (2014) exposed the terrestrial earthworm (Eisenia fetida) to 0.1, 1, 10, and 50 mg/kg DP for 14 days to examine lethality, oxidative damage, neurotoxicity, and transcriptomic profiles. Acute toxicity was low (no significant treatment effects on body weight or lethality at any treatment concentration); however, oxidative damage and effects on neuronal damage-related genes and pathways were reported.
Several soil toxicity tests for chlordane and mirex have been undertaken (Table 9-2), although most are several decades old and report different endpoints. Earthworm (Lumbricus terrestris) 5-day post wound healing was assessed after 10 to 30 days exposure to chlordane in artificial soil (Cikutovic et al.1999). The percent of worms completely healed 5 days post-wounding ranged from 75% to 81.2%, at the lowest concentration (6.25 μg/mg dw); no LOEL was determined. Survival (20 hour LD50) of field cricket (Gryllus pennsylvanicus) was reported as 0.89 ppm soil (assume 0.89 mg/kg dw), in a soil bioassay where crickets were placed on soil one hour after soil treatment with chlordane (Harris et al. 1964).
The knockdown dosage (KD30) and lethal dosage (LD50) values of mirex were determined for the land isopod (Armadillidium vulgare) and the soil millipede (Oxidus gracilis) by feeding with a diet containing different concentrations of technical mirex powder (Kuang and de la Cruz 1977). The KD30 and LD50 values (from food) for A. vulgare at 10 days exposure were 4.1 ppm (assume equivalency to 4.1 mg/kg dw) and 35.2 ppm, respectively; and for O. gracilis, 2.7 ppm and 198.7 ppm, respectively. Rajanna and de la Cruz (1975) conducted a phytotoxicity study to examine the effects of mirex on germination, emergence, and growth of seedlings of commonly grown field and pasture crops (see Environment and Climate Change Canada 2015 for species list). Total germination, seedling emergence, and early growth were reduced in several plant species relative to the controls when exposed to concentrations at or above the lowest test concentration of 0.15 ppm soil (0.15 mg/kg dw). Significant mirex effects on seedling growth rate at 2 weeks (dry weight of I00 seedlings) were found for all 6 species; crimson clover, johnson grass, and annual rye grass showed significant reduction in growth rate at 0.15 ppm soil mirex (0.15 mg/kg); tall fescue and alfalfa at 0.30 ppm soil (0.30 mg/kg); and alsike clover at 0.70 ppm soil (0.70 mg/kg).
|Substance (DP, Mirex, Chlordane)||Test Organism||Test Type||Endpoint||Value|
(mg/kg dw, unless otherwise stated)
|DP||Eisenia fetida||Acute toxicity, oxidative stress, neurotoxicity||Lethality/Body weight||greater than 50||Zhang et al. 2014|
|DP||Eisenia fetida||Acute toxicity, oxidative stress, neurotoxicity||oxidative stress markers, enzyme activity||0.1- 50 (varies with test)||Zhang et al. 2014|
|Prolonged soil toxicity:|
|5d post- wound healing|
|less than 6.25 (81.2% worms healed)||Cikutovic et al.1999|
|Prolonged soil toxicity:|
|5d post- wound healing|
|less than 6.25 (75.0% worms healed)||Cikutovic et al.1999|
|Prolonged soil toxicity:|
|5d post- wound healing|
|less than 6.25 (80.9% worms healed)||Cikutovic et al.1999|
|Soil toxicity: survival||20h LD50a||0.89 ppm soilb||Harris et al. 1964|
|Mirex||Isopod (Armadillidium vulgare)||Soil toxicity, dietary exposure||10d KD30||4.1 ppm||Lue and de la Cruz 1977|
|Mirex||Isopod (Armadillidium vulgare)||Soil toxicity, dietary exposure||10d LD50||35.2 ppm||Lue and de la Cruz 1977|
|Prolonged soil toxicity: dietary exposure||10d KD30||2.7 ppm||Lue and de la Cruz 1977|
|Prolonged soil toxicity: dietary exposure||10d LD50||198.7 ppm||Lue and de la Cruz 1977|
|Mirex||crimson clover (Trifolium|
(Sorghum halpense, annual rye grass (Lolium multiflorum)
|Prolonged soil toxicity: seedling growth rate||2 week signicant reduction seedling growth rate||less than 0.15c||Rajanna and de la Cruz 1975|
Abreviations: LD = lethal dose; KD = knockdown dose
a Reported as broadcast dosage need to kill 50% of worms by pesticide; presented as LD50 in this assessment.
b Not clear if ppm represents mg/L solution added to soil, or mg/kg soil dw.
c Reported in study as mg/kg soil, converted for this assessment.
For the purposes of this assessment, based on available endpoints from soil toxicity studies, the concentration of less than 0.15 mg/kg dw (2-week reduced growth rate for 3 plant species) is selected as the CTV based on analogue mirex, representing the lowest, prolonged study value. Given the uncertainty related to inter-/intra-species variation (2 trophic levels covered by analogue data) for chronic endpoints, an assessment factor of 10 is applied. Furthermore, given the CTV is an unbounded value (i.e., effects at lowest concentration in test), an additional assessment factor of 10 is applied. The resulting CTV is 0.0015 mg/kg dw. When this value is adjusted from test organic carbon content (0.04%) to standard soil (2% organic carbon content; ECHA 2010), the PNEC for soil organisms is 0.075 mg/kg dw. As the test soil for this latter study contained 0.04% OC, the maximum solubility of DP (based on analogue mirex) was ~ 31.7 mg/kg dw.
9.1.4 Empirical Studies in Wildlife
There are limited DP studies relevant to wildlife. Standard mammalian (rodent) repeated dose toxicity studies conducted with DP have generally shown no adverse effects up to the highest dose tested: e.g., 5000 mg/kg-bw/day (90-day rat study, Oscarson 1975; 28 day oral dose/reproductive rat study Brock et al. 2010). At the highest administered dose of 25 000 mg/kg-bw/day, DP had no effect on Sherman-Wistar rats in an acute oral study and the NOAEL after 90 days in a repeated (sub-chronic) study was 100 000 ppm (USEPA 2008, cited by Crump et al 2011). See the Human Health Effects section for detailed analysis of other rodent and other mammal toxicity studies.
Crump et al. (2011) studied concentration-dependent effects of DP using in vivo and in ovo toxicity approaches in domestic chicken (Gallus gallus domesticus) embryonic hepatocytes and chicken embryos. DP was injected to eggs prior to incubation, and monitored until pipping (or day 22). No overt toxic effects were observed up to the maximum dose of 3uM in hepatocytes, and up to the highest nominal DP dose (500 ng/g/egg) for pipping success. Furthermore, no changes to the mRNA transcript levels of the target genes were observed, despite the target genes responding to other flame retardants (e.g., HBCD) in earlier studies. The authors concluded that DP did not significantly affect cytotoxicity or embryonic viability in the chicken at concentrations 10 times greater than those detected in herring gull eggs in the Great Lakes. However, a shift in isomeric content of syn- and anti-DP was detected between stock solutions and hepatic tissue; the proportion of syn-DP increased from 0.34 to 0.65, as anti-DP decreased (0.66 to 0.35).
Li et al. (2013) studied the effect of DP on male common quails (Corturnix coturnix) continuously exposed to commercial DP by gavage for 90 days at dose concentrations ranging from 1 to 100 mg/kg bw/d. Liver enzyme activity and oxidative stress were measured. The authors reported DP effects on some measures of enzyme activity (e.g., significant decrease of PROD in all exposed groups relative to the control, significant increase in ERND and the antioxidant enzyme catalase in high exposed groups relative to control). Furthermore, the study found DP was more prone to accumulate in liver (vs. serum, muscle), and syn-DP accumulated (vs. anti-DP) in the high-exposure DP groups.
|Test Organism||Test Type||Endpoint||Value||Reference|
|domestic chicken (Gallus gallus domesticus)||wildlife toxicity: pipping success||90 d|
Cytotoxicty, Pipping success, mRNA expression
|greater than 500 ng/g egg (nominal dose),|
greater than 3 uM for cytotoxicity
|Crump et al. 2011|
|Wildlife Sub-chronic-||90 d enzyme activity measures||1 mg/kg bw/d||Li et al. 2013|
The existing mammalian and avian studies suggest that although DP may be bioavailable to wildlife, it is not overtly toxic (no effects at highest dose). However, the recent molecular level avian studies suggest DP may affect enzyme activity levels in test species, although the ecological relevance of these results is not clear.
A CTV of 5000 mg/kg bw/day (no adverse effect up to the highest dose = 5000 mg/kg bw/day) from Brock et al. (2010) was selected from a range of laboratory rodents tests, based upon a 28-day combined repeated-dose and reproductive toxicity study conducted according to OECD guidelines (see Health Assessment Section). This NOAEL was supported by an older, unpublished 90-day oral dose study (Oscarson 1975). Assuming exposure to small mammals like voles and shrew, the Wildlife Toxicity Reference Value (TRV) approach (Sample et al. 1996) was used to normalize effects in rats to a typical body weight of a shrew, which represents a surrogate wildlife species for mammals consuming soil organisms (see Environment and Climate Change Canada 2015 for input values), resulting in a TRV of 11798 mg/kg bw/day. An assessment factor of 10 was applied to account for extrapolation from laboratory to field conditions. The resulting shrew TRV was 1179.8 mg/kg bw/day.
Assuming exposure to wildlife, the same CTV of 5000 mg/kg bw/day (no adverse effect up to the highest dose = 5000 mg/kg bw/day) from Brock et al. (2010) was selected to determine the TRV for wildlife (piscivores). The Wildlife Toxicity Reference Value (TRV) approach (Sample et al. 1996) was used to normalize effects in rats to a a typical body weight of mink (Mustela vison) and river otter (Lontra canadensis) respectively, which represent surrogate wildlife species, resulting in TRV wildlife estimates of 3769 and 2288 mg/kg bw/day (see Supporting Documentation, Environment and Climate Change Canada 2015 for input values). An assessment factor of 10 was applied to account for extrapolation from laboratory to field conditions. The resulting wildlife TRV was 228.8 (otter) to 376.9 (mink) mg/kg bw/day.
9.2 Ecological Exposure Assessment
While measured DP concentrations in the environment have been presented, limited data concerning concentrations of DP in water in Canada have been identified. Therefore, environmental concentrations have been estimated from available Canadian information, including estimated substance quantities, estimated release rates, and characteristics of the receiving environment. Environmental concentrations have been estimated for industrial release scenarios, as described below.
9.2.1 Exposure Scenarios and Predicted Environmental Concentrations
The aquatic exposure to DP is expected if industry (e.g., manufacture, formulation) releases DP either directly or to a wastewater treatment system that discharges its effluent to water. The concentration of the substance in the receiving water near the discharge point of the wastewater system is used as the predicted environmental concentration (PEC) in evaluating the aquatic risk of the substance. It can be calculated using the equation:
PEC= [1000 × Q × L × (1 - R)] / [N × F × D]
- aquatic concentration resulting from industrial releases, mg/L
- total substance quantity used annually at an industrial site, kg/yr
- loss to wastewater, fraction
- wastewater system removal rate, fraction
- number of annual release days, d/yr
- wastewater system effluent flow, m3/d
- receiving water dilution factor, dimensionless
Several conservative aquatic industrial release scenarios were developed to cover a range of known DP industrial activities that could occur in Canada. The scenarios include: manufacturing of wires and cables, automobile manufacturing, and manufacturing of hard plastic connectors. Information from the different facilities considered was collected and scenarios reflected expected practices and conditions, including type of wastewater treatment, and direct or indirect releases to the receiving environment.
As some DP is imported in bulk in part of a liquid mixture which may generate residues in transport containers, container cleaning operations may lead to environmental releases of these substances. Although environmental concentrations of DP resulting from these releases may be high, these releases would likely be episodic in nature and probably of short duration. Given these considerations and the current data gaps associated with container cleaning operations and practices, a quantitative exposure characterization was not developed.
Table 9-5 presents the range of inputs used to estimate the resulting aquatic concentrations close to the industrial point of discharge. Based on these assumptions, these industrial scenarios yield predicted aquatic environmental concentrations (PECs) of 4.38 x 10-8 to 1.2 x 10-5 mg/L for total (dissolved and particle associated) DP. Predicted PEC values that exceeded DP water solubility (2.85 x 10-7 mg/L) were replaced by this value (i.e., aquatic PECs were capped at the water solubility limit). The aquatic PEC value represents the level of exposure in the receiving water near the point of the discharge at each site.
|Input||Value||Justification and reference|
|Quantity used per site (kg/yr)||less than 10 000||Range includes site quantities identified in a section 71 survey or EC assumptions determined from sec. 71 data|
|Loss to wastewater (%)||0.01 to 1.0||OECD 2004, 2009|
|Wastewater system removal efficiency (%)||60 and 94||Predicted for primary and secondary treatment (STP Model 2.1, highest removal rate of 4 models)|
|Number of annual release days (days)||250 to 350||EC standard assumption for continuous releases|
|Wastewater system effluent flow (m3/d)||14 024 to 65 700||Site specific wastewater treatment system data|
|Dilution factor (–)||1 to 10||Site specific wastewater treatment system flow rate/receiving environment flow rate. When a dilution factor was greater than 10, a maximum default value of 10 was used.|
An equilibrium sediment-water partitioning approach was used to estimate the concentration of DP in bottom sediment. This approach is based on a partitioning principle described by the European Chemicals Agency (ECHA 2010) and incorporates two additional calculation methods. The first method is to estimate the substance's concentration in the aqueous phase (dissolved) of the overlying water from its total concentration, according to studies by Gobas (2007 and 2010). The second method is to estimate a substance's concentration in bottom sediment from its concentration in the aqueous phase of the overlying water based on an equilibrium partitioning assumption between bottom sediment and overlying water described by the USEPA's National Center for Environmental Assessment (US EPA 2003). At equilibrium, the predicted environmental concentration (PEC) in bottom sediment can linearly correlate with the concentration in the aqueous phase of the overlying water. Sediment exposure scenarios were developed as an extension of the industrial aquatic release scenarios described above to determine equilibrium sediment PECs, standardized to 4% organic carbon (a typical organic carbon content in bottom sediment for rivers and lakes) (Webster et al. 2004). The resulting PEC in bottom sediment ranged from 1.5 to 10 μg/kg dw (0.0015 to 0.010 mg/kg dw).
The sediment PEC is similar to the maximum DP concentration in tributary sediment measured for Lake Superior (1.6 μg/kg dw, percent organic carbon not specified; Shen et al. 2011b), and much greater than DP reported for Lake Winnipeg sediment (0.03 μg/kg dw, Tomy et al. 2007). The sediment PEC value is, however, lower than the highest DP concentrations measured in sediments taken from the Niagara River (flowing between Lakes Erie and Ontario) and Lake Ontario, where DP was measured in all samples in studies by Shen et al. (2011a, b) and Sverko et al. (2008), up to 310 μg/kg dw (in a Niagara River tributary) and 586 μg/kg dw (open water sediment Lake Ontario) (percent organic carbon for the sample is not provided). The Niagara River has a long history of industrial activity and related discharges, including the manufacture of DP at Niagara Falls, New York, which has influenced the relatively high chemical concentrations along the river and in Lake Ontario (Shen et al. 2011a, b). The degree to which the DP source in these areas originated in Canada is not clear.
An approach described by the ECHA (2010) was used to estimate predicted environmental concentrations in soil (soil PECs) resulting from the land application of wastewater biosolids. This approach employed the quantity of biosolids accumulated within the top 20 cm layer (ploughing depth) of soil over 10 consecutive years as the basis for soil PECs. The underlying assumption of the approach was that substances were subject to no loss due to volatilization, leaching and soil run-off upon their entry into soil via biosolids land application, however loss due to degradation was considered (half-life of 2 years in soil was assumed). Soil exposure scenarios were developed from the scenarios described above, using sludge concentration and production rates based on site specific waste water treatment plants. The estimated concentration in biosolids ranged from 0.012 mg/kg dw to 5.1 mg/kg dw, which is well above the current range of North American biosolid DP concentrations reported (0.009 to 0.050 mg/kg dw; see Environment and Climate Change Canada (2015), Davis et al. (2012)). Soil PECs were standardized to 2% organic carbon (ECHA 2010), and the resulting values ranging from 1.4 x 10-4 to 0.059 mg/kg dw.
A Wildlife Total Daily Intake (TDI) was derived for the shrew consuming soil organisms (worms) exposed to DP applied to soils as a function of biosolids application using the BASL4 model. By using the maximum predicted DP concentration in biosolids (5.1 mg/kg) and assuming application to soils once per year over 10 years (and degradation half-life of 2 years), the resulting TDI is 0.0716 mg/kg bw/day. This value is considered conservative because the BASL4 model does not consider metabolism in its estimate.
A Wildlife Total Daily Intake (TDI) for mink (Mustela vison) and river otter (Lontra canadensis) consuming fish were estimated following the approach of US EPA (1993). In calculating the TDI, a lake trout tissue concentration (Ci) of 0.00085 mg/kw (ww), was selected representing the highest published mean concentration of DP in Canadian biota (Ismail et al. 2006), resulting in a PEC of 1.1 x 10-4 (mink) to 1.2 x 10-4 (otter) mg/kg bw/day (see Environment and Climate Change Canada (2015) for details of TDI model inputs).
In addition to industrial sources of DP, consumer or commercial products can represent a source of DP to the environment (e.g., via volatilization and particulates from abrasion (ECB 2004)). Although there is almost no data quantifying releases from products available in the literature, the presence of DP in dust samples (see Human Health section 10.1.1.2), and wastewater treatment plant media (influent, effluent, and sludge) (Kolic et al. 2009; Davis et al. 2012; LaGuardia et al. 2012), support that the substance can be released from products (Davis et al. 2012). Few studies report on Canadian or North American DP concentrations in waste effluent and wastewater treatment system by-products (e.g. sludge/biosolids), however Kolic et al. (2009) reported both syn-DP and anti-DP were detected in sludge collected from a Toronto area wastewater treatment plant: approximately 100 ng/g and from 10 to 100 ng/g for syn-DP and anti-DP respectively (values read from log-scale graph). For dust, recent high-volume active air sampling at a semi-urban location in Toronto, Canada, determined DP was one of the most frequently detected OFRs (Shoeib et al. 2014), suggesting non-point diffusive sources. Similarly, other studies in Canada have attributed the detection of DP in household dust to DP-containing consumer products, as there were no nearby DP manufacturing facilities (e.g. Zhu et al. 2007). Median dust levels of DP were the second highest relative to other non-PBDE brominated flame retardants measured in the recent Canadian Household Dust Survey of 413 homes (see Human Health section 10.1.1.2), although median DP was lower than dust concentrations of organic phosphate based flame retardants in the same study. A 95th percentile concentration of 107.4 ng/g for Canadian household dust was determined for this assessment (see Human Health Section 10.1.1.2), which suggests DP release from this route is measureable.
While service life release rates were not found for DP, a study by Kemmlein et al. (2003) determined specific emission rates (SER) of 0.3 ng/m2/h for decaBDE (from OctaBDE mixture) during a 105-day test of television set housing (23 ᵒC). OECD (2009) identifies potential volatility to atmosphere from service life for generic OFRs in plastics, estimated at 0.05% over lifetime for indoor or outdoor use; however, this generic value may be an overestimate for a very low volatility OFR like DP. Environmental release of the substance from plastic polymers via leaching is considered possible, albeit low, as many products identified with DP will not be in contact with water on a regular basis (e.g., electronics). The potential release of OFRs from plastics during service life to water is estimated at 0.05% over lifetime if the substance is for indoor use or 0.16% over service life if use is outside (OECD 2009a). The large majority of products would be enclosed/indoor use therefore the release rate of 0.05% is more likely (OECD 2009a). A coarse scenario for the diffuse release of DP from commercial and consumer products in Canada was determined, assuming the indoor release rate of 0.05% per year over service life from OECD (2009a). Using the upper range import quantity for Canada in 2011 (10 000 kg), it was assumed the entire quantity was used in products; resulting in a release estimated at 5 kg. This scenario includes a number of assumptions: the maximum values from range of import, complete use of DP in products, that all use in products in Canada is known and reported, low exposure to water over the service lifetime, and indoor use. This result suggests that significant release of DP from commercial and consumer products is unlikely. However, the scenario result is considered to be highly uncertain.
Overall, releases from commercial and consumer products are expected to be geographically dispersed and spread out over the duration of the service life and end of life stages. While the scenario presented above may provide coarse estimate of release to the environment during service life of commercial and consumer products, there is an absence of data to quantitatively address solid waste disposal of dust and end of life releases from all manufactured items, including non-residential sources.
9.3 Characterization of Ecological Risk
9.3.1 Risk Quotient Analysis
A risk quotient analysis, integrating conservative estimates of exposure with toxicity information, was performed for the sediment and soil media, as well as for wildlife, to determine whether there is potential for ecological harm in Canada. A risk quotient analysis was not conducted for the aquatic environment due to the low likelihood of DP exposure via water and unreliable aquatic toxicity data. The aquatic CBR (discussed above) suggests low likelihood of lethality to aquatic organisms through water exposure; however, sublethal effects cannot be ruled out.
The site-specific industrial scenarios presented above yielded a predicted environmental concentration (PEC) of 4.38 x 10-8 to 2.85 x 10-7 mg/L for total DP (capped at water solubility limit). This PEC value represents the level of exposure in the receiving water near the point of the discharge. Using the aquatic PEC in water to determine equilibrium sediment PECs, standardized to 4% OC, the resulting sediment PEC is 0.0015 to 0.010 mg/kg dw (or 1.5 to 10 μg/kg dw). A predicted no-effect concentration (PNEC) of 0.0171 mg/kg dw (17.1 μg/kg dw) was derived from a chronic marine sediment organism toxicity study for analogue chlordane (see Ecological Effects section). The resulting risk quotient (PEC/PNEC) = 0.09 to 0.57. Therefore, harm to sediment organisms is unlikely for these industrial scenarios, although at higher PEC values the RQ approaches the range of potential concern. Furthermore, if considering upper range sediment DP concentrations measured in the southern Great Lakes region, (e.g., 2.23-586 μg/kg dw for surficial sediment concentrations in Lake Ontario in samples collected in 1998 (Sverko et al. 2008)), the risk to sediment organisms could be greater.
Using a similar risk quotient approach, predicted soil PECs resulting from biosolid applications to land (standardized to 2% OC) ranged from 1.4 x 10-4 to 0.059 mg/kg dw. The PNEC for soil organisms (based on plant toxicity values for analogue mirex) is 0.075 mg/kg dw (See Ecological Effects section). The resulting risk quotients (PEC/PNEC) are 0.002 to 0.78. This suggests harm to soil organisms is currently unlikely for these scenarios. However, it is noted that in at least one scenario, the risk quotient is close to 1, therefore a change in factors contributing to a higher soil PEC (e.g. much larger quantity used, or soil degradation half-life greater than assumed 2 years), could result in possibility of risk to soil organisms.
A Wildlife TDI was derived for the shrew consuming soil organisms (worms), using the BASL4 model, which calculates soil DP from wastewater biosolids applied to land. In calculating the TDI, the maximum predicted DP concentration in biosolids was assumed (5.1 mg/kg), over a 10-year exposure, resulting in a TDI of 0.0716 mg/kg bw/day. The derived TRV was 1179.8 mg/kg bw/day (see Ecological Effects Assessment section). The resulting risk quotient result (TDI/TRV) is 6.1 x 10-5 for shrew, indicating that even with conservative assumptions, current DP concentrations in Canadian biota are unlikely to exceed minimum effects levels (Table 9-6).
The Wildlife piscivore TDI was derived for mink (Mustela vison) and river otter (Lontra canadensis) consuming fish following the approach of US EPA (1993). A lake trout tissue concentration (Ci) of 0.00085 mg/kw (ww), was selected representing the highest published mean concentration of DP in Canadian biota (Ismail et al. 2006), resulting in a TDI of 1.1 x 10-4(mink) to 1.2 x 10-4 (otter) mg/kg bw/day (see Supporting Documentation, Environment and Climate Change Canada 2015) for details of TDI model inputs). The derived TRV was 376.9 (mink) and 228.8 (otter) mg/kg bw/day (see Ecological Effects Assessment section). The resulting risk quotient results (TDI/TRV) are 2.93 x 10-7 for mink and 5.08 x 10-7 for otter, indicating that even with conservative assumptions, current DP concentrations in Canadian biota are unlikely to exceed minimum effects levels (i.e., by 10 000 fold margin) (Table 9-6).
|Media||Scenario||PNEC or TRV||PEC or TDI||RQ|
|Sediment||Industrial release to water||0.0171 mg/kg dw||0.0015 to 0.010 mg/kg dw||0.09 to 0.57|
|Soil||Biosolid application to soil||0.075 mg/kg dw||1.4 x 10-4 to 0.059|
|0.002 to 0.78|
|Shrew (consuming worms;10-year exposure)||1179.8 (shrew) mg/kg bw /day||0.0716 mg/kg bw /day||6.1 x 10-5|
|Piscivore (mink and otter/fish)||376.9 (mink)|
228.8 (otter) mg/kg bw /day
|1.1 x 10-4 (mink)|
1.2 x 10-4 (otter) mg/kg bw /day
|2.93 x 10-7 (mink)|
5.08 x 10-7 (otter)
9.3.2 Consideration of Lines of Evidence and Conclusion
DP is expected to be persistent in water, soil and sediment. DP is predicted to have moderate to high bioaccumulation potential, and the substance is found widespread in biota at high concentrations (e.g., up to greater than 100 μg/kg dw), suggesting bioaccumulation is occurring. Although there is low importation quantity of DP into Canada, certain regions of Canada (Great Lakes region) are experiencing high environmental concentrations of DP, potentially due to proximity to DP manufacturing sources in the Great Lakes region (e.g., Niagara Falls, New York). DP is a High Production Volume substance in the USA, and therefore environmental transport of DP may occur from the northern USA to Canada, resulting in higher exposure and risk for Canadian organisms in certain regions of Canada (i.e., southern Great Lakes), than predicted by some exposure estimates based on Canadian usage. This information, along with information on its uses, indicates potential for release into the Canadian environment. Once released into the environment, DP will be found mainly in sediment and soil, where it may persist for long periods of time. Based on measurements in remote regions and modelling results, DP, via sorption to particles, also has potential for long-range transport and deposition in remote areas.
DP ecotoxicity studies are lacking. The DP ecotoxicity analogues used in this assessment, chlordane and mirex, demonstrate potential for toxicity to sediment and terrestrial organisms. However, as these substances are more bioavailable than DP, they are conservative and represent more toxic analogues for determining PNECs. Using these conservative PNECs, the risk of harm based on current DP import quantities is predicted to be low (although approaching risk to soil organisms at one scenario). However, due to the possibility of exposure from DP sources in the Great Lakes region, as well as the uncertainty relating to potential increases in DP usage within Canada (e.g., as an alternative flame retardant to DecaBDE in a range of flame retardant applications of electronic wiring and cables, automobiles, plastic roofing materials, and hardplastic connectors), precaution is warranted.
Additionally, although not evaluated in this assessment, considering the detection of other "declorane-related" analogues (e.g., Dec 602, Dec 603, Dec 604, CP) in the Canadian environment and in biota at concentrations in the range of DP (or in some cases higher), the potential for cumulative effects of similar "Dechlorane Plus-like" substances should be considered. DP-related compounds also include impurities formed through side reactions in DP synthesis (e.g., 1,4-DP, VCH-DP, 1,3- DPMA, 1,5-DPMA ) (Sverko et al. 2010). Information on production, applications, physical-chemical properties, and toxicity are lacking for all these substances, but initial studies indicate they may have similar P and B characteristics to DP (Sverko et al. 2011).
This information indicates that DP does have the potential to cause ecological harm in Canada.
9.3.3 Uncertainties in Evaluation of Ecological Risk
A lack of empirical data and a reliance on modelling contributes to several sources of uncertainty for the ecological assessment of DP. Estimation of physical chemical property data largely required the use of (Q)SAR models and while attempts were made to keep these properties internally consistent, due to its very hydrophobic nature, the substance can be problematic for modelling. Consequently, there is a moderate level of confidence with properties that govern bioavailability and uptake (e.g., partition coefficients), particularly those used for further exposure and toxicity modelling (i.e., log Kow).
There is high confidence that DP is very stable in the environment with a long residence time and will bioaccumulate in organisms from exposures via the food web or direct contact with soil or sediment rather than from water.
Limited empirical BAF data lead to the use of mass-balance models for supporting information. These models are highly sensitive to log Kow error, metabolism rate error and dietary assimilation efficiency. BAF modelling is thus regarded with a low to moderate level of confidence.
PEC estimates in soil and sediment are mass-balance model based. Several of the model parameters are known to be variable (emission factors, removal rates in WWTPs, sludge adsorption, effluent release limits) and thus contribute to a range of predicted environmental concentrations. It is difficult to quantify the impact of the uncertainty of these parameters on soil PEC estimates, as there is as of yet no reliable Canadian monitoring data from near field emission sources for DP soil comparison purposes. Consequently, conservative estimates of the PEC are used for further exposure analysis to wildlife, but overall there is a low to moderate level of confidence with the emission scenarios used to generate PEC values.
Exposure scenarios for use in risk analysis were developed based on the best available information, and they are considered sufficiently protective to characterize potential risks from releases from the use of DP to the Canadian environment. Even with conservative assumptions of DP quantities in use at industrial sites, risk quotients were much less than one for sediment and most soil scenarios, suggesting low to no current risk for these organisms. However, the risk quotient approached one for at least one soil scenario (risk quotient = 0.78). Uncertainty in this scenario relates to factors described in soil PEC estimates above.
There is limited information characterizing potential releases from products in use and during disposal/recycling of at the end of their service life. Furthermore there is uncertainty with respect to the quantity of products in use. While a coarse exposure scenario was developed which suggested low quantities for dispersed release of DP from commercial and consumer products, this area of the assessment represents an area of uncertainty of DP exposure to organisms in Canada.
Additionally, DP is a High Production Volume substance in the USA; therefore, environmental transport of DP may occur from the northern USA to Canada, resulting in higher exposure for Canadian organisms than determined by some exposure estimates based on current DP use in Canada. Due to the uncertainty surrounding DP exposure levels in Canada, follow-up monitoring is recommended.
An important area of uncertainty relates to the data gaps for DP toxicity to sediment-dwelling and terrestrial organisms. There are limited analogue options for DP, given that the most similar chemical analogues to DP also lack ecotoxicity data for soil and sediment. Using chlordane and mirex as analogues is likely conservative (i.e., the analogues are likely more toxic), as they are considered more bioavailable than DP. Despite similar predicted modes of action among these substances, the analogue substances are not a 1:1 read-across for similarity with DP, therefore differences in degree of toxicity between DP and these analogues is likely. Furthermore, toxicity studies for these analogues are several decades old and details are limited, contributing uncertainty to study results. There is low to moderate level confidence with the soil and sediment toxicity data in this assessment.
Limited short-term mammalian repeated oral dose toxicity data were available for DP for wildlife toxicity assessment. Therefore, there is a moderate level confidence with the mammalian toxicity data used for wildlife assessment.
Finally, while assessment of "dechlorane-related" substances (e.g., Dechlorane 602, Dechlorane 603, Dechlorane 604 etc. described in section 2.2 of Substance Identity) is beyond the scope of the current screening assessment, data for environmental media and biota concentrations, as well as data for fate and behaviour and toxicity (including the potential for cumulative exposure and effects with DP) is important to understand how similar these substances are to DP.
10. Potential to Cause Harm to Human Health
10.1. Exposure Assessment
This exposure assessment is based on the total exposure of DP isomers (i.e., the combined exposures to both the anti-DP and syn-DP isomers). Therefore only the sum of DP isomers is reported and used to derive estimates of intakes of DP
10.1.1 Environmental Media and Food
Based on its very low water solubility and low to very low vapour pressure, DP is expected to partition predominantly to particles, dust and sediment when released in the environment. DP is identified as a highly sorptive substance, sorbing to particles in air (see section 8).
Canadians may be exposed to DP in air, dust, soil, sediment, water and food, including breast milk. Conservative estimates of daily intake of DP are presented in Appendix C. For all age groups, the main contribution to the estimated daily intake was from food, followed by the ingestion of dust and the inhalation of air particles. DP exposure via the ingestion of water and soil was found to be very low and was considered negligible. The highest estimate of daily intake was 8.2 ng/kg-bw/d for young children (0.5 to 4 years of age).
10.1.1.1 Ambient Air
DP has been monitored in ambient air in Canada and elsewhere (see section 7). Hoh et al. (2006) were the first to report the occurrence of DP in the environment in 2004 at IADN sites along the Great Lakes, where DP was frequently detected (greater than 90%) in outdoor air. DP concentrations at the Canadian site (Point Petre, a rural site) were measured up to 21 pg/m3 (n=12). However, DP concentrations were highest (max of 490 pg/m3) at the Sturgeon Point, New York (NY), site (polulation 10 000), with a median of 2.5 pg/m3. The authors noted that the elevated concentrations at Sturgeon Point, a relatively rural site, were possibly associated to emissions from the nearby DP manufacturing facility in Niagara Falls, NY (Hoh et al. 2006). Other IADN studies have been published since 2006 and have also observed higher DP levels at the Sturgeon Point site (Venier and Hites 2008; Salamova and Hites 2011; Venier et al. 2015). Salamova and Hites (2011) did not observe any significant changes in air concentrations of DP from 2005 to 2009; however, the authors observed a significant increase in DP concentration with increasing distance to the manufacturing plant in Niagara Falls, NY (Salamova and Hites 2011).
In a separate air monitoring study in the Great Lakes region, conducted by Environment and Climate Change Canada (spanning 2008-2010), the highest DP concentrations were measured up to 340 pg/m3 in samples (n=30) collected at the Burnt Island, Ontario site (Hung et al. 2014).
Two recent ambient air monitoring studies conducted in Toronto, Canada, showed that DP was frequently detected (greater than 90%), and concentrations were measured up to 7.5 pg/m3 (n=70) for samples collected between 2010 and 2012 (Diamond et al. 2013; Shoeib et al. 2014).
In addition to Canadian monitoring in the Great Lakes region, DP has also been monitored in Alert, NU, a remote location in the Canadian High Arctic (Xiao et al. 2012), and the northernmost inhabited location in the world (Iqaluit 2010), where concentrations were found up to 2.1 pg/m3 in samples (n=14) collected in 2006-07, indicating that DP may be available for long-range atmospheric transport (see section 8.1.1).
The maximum concentration of 340 pg/m3 measured in Canada in the Great Lakes area by Environment and Climate Change Canada (Hung et al. 2014) was selected for deriving daily intakes of DP in ambient air for the general population in Canada. This value is considerably higher than those at other Canadian locations (e.g., Hoh et al. 2006; Shoeib et al. 2014), and is similar to the highest concentrations measured at the IADN Sturgeon Point site (Hoh et al. 2006) near a point source. These estimates are expected to represent general population exposures, including northern populations, given the lower levels measured in the Canadian North (i.e., Xiao et al. 2012).
10.1.1.2 Indoor Air
No studies on DP monitoring in Canadian indoor air were identified.
Cequier et al. (2014) investigated the occurrence of DP and 36 other flame retardants in Norwegian households (n=48) and classrooms from two primary schools (n=6). DP isomers were detected in 4% of residential samples but were not detected in the school classrooms. Concentrations in residential living rooms ranged from not detected (mdl not specified) to 15 pg/m3 greater than (sum of the individual isomeric maximum values). An additional study in Europe was identified that measured DP in a limited number of indoor air samples (n=3) (specific indoor environment not specified) in 2009 from Norway; however, DP was not detected (detection limit of 16 pg/m3 greater than ) (TemaNord 2011). Therefore, the upper-bounding concentration of DP selected for ambient air, i.e., the maximum concentration of 340 pg/m3 measured in Canada in the Great Lakes area by Environment and Climate Change Canada (Hung et al. 2014), was selected for deriving daily intakes of DP in indoor air for the general population.
DP is ubiquitous in house dust based on its high detection frequency in several Canadian and international house dust studies (Appendix D).
DP was targeted in the Canadian baseline study of halogenated flame retardants in household dust (n=413) collected in 2007-2008 from various Canadian cities within the Canadian House Dust Study (CHDS) (CHDS preliminary data; Kubwabo et al., manuscripts in preparation, Environmental Health Science and Research Bureau (EHSRB), Health Canada; unreferenced, dated November 21, 2013). DP was detected in 94% of samples, and concentrations ranged from not detected (mdl = 1.53 ng/g) to 2508 ng/g, with a median and 95th percentile of 11.5 ng/g and 107.4 ng/g, respectively.
DP was also detected (greater than 90%; n=20) in samples collected in 2012 across the Greater Toronto Area (GTA), ON, where concentrations ranged from not detected (detection limit = 4 ng/g) to 170 ng/g, with a mean of 34.5 ng/g (Diamond et al. 2013). DP was detected in all house dust samples collected during 2007-08 from homes (n=116) in Vancouver, Canada (Shoeib et al. 2012). Samples were collected from houses of female participants in the Chemicals, Health and Pregnancy (CHiRP) study. DP concentrations in this study ranged from not detected (detection limit = 0.8 ng/g) to 354 ng/g with a median of 6.8 ng/g and a 95th percentile of 57.7 ng/g (Shoeib et al. 2012). In a separate study, DP was also detected in all dust samples collected in Canadian households in Ottawa, ON in 2002-03 (n=69) at concentrations ranging from 2.3 to 182 ng/g with the exclusion of one sample measured at 5683 ng/g that could not be explained based on questionnaire responses (Zhu et al. 2007). The median and 95th percentile for this sample set were 14 and 121 ng/g, respectively. As there are no nearby DP manufacturing facilities in Ottawa, the authors noted that the detection of DP in household dust was likely due to DP-containing consumer products.
DP has also been found to be ubiquitous in US house dust, and concentrations are generally similar to those measured in Canada. DP was detected in all samples collected from living area surfaces of 16 homes in California in 2006 and 2011, with concentrations ranging from 3 to 47 ng/g (median up to 10 ng/g) (Dodson et al. 2012). DP was also detected in all dust samples (n=38) collected in 2002-03 from vacuums collected from homes in Boston, Massachusetts, where DP concentrations were found up to 111.5 ng/g, with a median of 13.1 ng/g (Johnson et al. 2013).
DP has also been targeted for analysis in dust from aircraft cabins. Allen et al. (2013) sampled dust from 19 commercial aircrafts manufactured between 1986 and 2003 by Boeing, Airbus, Canadair Regional, McDonell Douglas or Embraer. Dust samples were collected from the 19 aircrafts, with one aircraft being sampled twice, for a total of 40 samples, although the sampling year and airport location were not specified. DP was detected in 100% of samples. For dust collected from aircraft carpeting, DP concentrations ranged from 132 to 13 700 ng/g with a median of 440 ng/g, while for dust collected from air vents, DP ranged from 65 to 11 800 ng/g with a median of 460 ng/g (Allen et al. 2013).
The DP 95th percentile concentration (107.4 ng/g) from the Canadian baseline study was selected for deriving intake estimates of DP via dust ingestion for the general population in Canada. Although concentrations in certain environments (e.g., aircrafts) may be higher, the overall magnitude of these exposures (frequency and duration) are expected to be lower than those conservatively estimated for daily exposure based on household dust for the general population of Canada.
10.1.1.4 Soil and Sediment
No monitoring data on DP in soil in Canada were identified (see section 7). However, several studies have monitored DP in sediments in the Great Lakes region (Sverko et al. 2008; Sverko et al. 2010; Shen et al. 2010; Shen et al. 2011). For example, DP was detected in all surficial sediment samples collected in the Great Lakes from 2001-07, and concentrations of total DP concentrations ranged from 0.014 to110 ng/g dw, the highest concentrations being associated to Lake Ontario (Shen et al. 2010).
A maximum DP soil predicted environmental concentration (PEC) of 59 000 ng/g dw (0.059 mg/kg dw) was estimated for land application of biosolids on an agricultural field using conservative approaches (see section 9.2.1). As no appropriate or relevant monitoring studies on DP in soil were identified, the soil maximum PEC (59 000 ng/g dw) was selected for upper-bounding intakes from the ingestion of soil for the general population in Canada.
10.1.1.5 Drinking Water
No studies were identified that reported DP in drinking water from Canada or elsewhere. However, DP has been monitored in surface water in Canada and elsewhere (see section 7). Great Lakes monitoring studies have shown that DP concentrations are highest, up to 14 pg/L, in Lake Ontario in monitoring spanning 2005-12) (Muir et al. 2011 and 2014; Venier et al. 2014). As no drinking water data were available, drinking water exposure was characterized using surface water monitoring. Upper-bounding daily intakes of DP for the general population of Canada were based on the highest DP concentration (only mean reported; 14 pg/L), measured in Lake Ontario (Venier et al. 2014). The use of upper-bounding surface water concentrations is considered to be a conservative surrogate for estimating exposure from drinking water, as no removal from drinking water treatment is accounted for.
No studies were identified that reported DP in marketed foods in Canada. However, DP has recently been measured in three categories of baby food (formula, cereal, and purée) bought in the U.S. and China in 2013 (Liu et al. 2014). DP concentrations in formula (n=12), cereal (n=15) and purée (n=8) from the U.S. were measured up to 83.2, 427 and 23.6 pg/g ww, respectively. DP concentrations in formula (median of 16 pg/g fresh weight) were an order of magnitude higher than those recently reported for breast milk (median of 1.6 pg/g fresh weight) from nursing women in Canada (Zhou et al. 2014; ww concentrations obtained from personal communication with Environmental Health Science and Research Bureau (EHSRB), Health Canada, dated May 15, 2014; see section 10.1.1.7). Of note, the maximum cereal sample concentration (427 pg/g ww) collected in the U.S. was the second highest total DP level in the Liu et al. (2014) study. Based on analyses of both food and packaging, the authors concluded that it was unlikely that the samples were contaminated from their packaging. Rather, the authors suggested that raw food material itself may have been contaminated or that it became contaminated during production (Liu et al. 2014). Similarly, in a separate market basket study conducted in Japan DP was present in four food groups (i.e., sugar and confectionary; legumes and their products; fish, shellfish and their products and; meat and eggs) at concentrations ranging from 1.5 to 3.3 pg/g ww (Kakimoto et al. 2014). The authors noted that contamination may have been from the raw materials such as wheat and sugar or by contamination through the confectionary making process (Kakimoto et al. 2014). Fish concentrations measured in the Japanese study were lower than levels reported by Ismail et al. (2009) for Lake Ontario lake trout. In an earlier study, DP was measured in halibut from Greenland (0.44 ng/g) marketed for Japanese consumption (Kakimoto et al. 2012). Finally, a previous study suggested that vegetables, grains, and fish from a DP polluted area of China were highly contaminated by DP, with total DP concentrations ranging from 56.8 for loach to 2700 pg/g for green onion (Hoh et al. 2006).
DP has been reported in biota in Canada, and several studies have reported on the detection of DP in fish the Great Lakes region (Hoh et al. 2006; Shen et al. 2010; Ismail et al. 2009; Muir et al. 2011; Muir et al. 2014). The highest concentrations were associated with archived samples of lake trout collected in Lake Ontario from 1979-2004. Mean DP concentrations peaked in 1988 at 7.2 ng/g lw (0.85 ng/g ww), and decreased in subsequent years to 2.3 ng/g lw (0.31 ng/g ww) in 2004 (Ismail et al. 2009). In contrast, in a separate study, DP concentrations ranged from 0.14-0.91 ng/g lw in archived walleye fish samples collected from 1980 to 2000 from Lake Erie, USA, and did not show an increase or decrease over time (Hoh et al. 2006). DP fish monitoring of samples collected from Lake Winnipeg between 2000 and 2003 by Tomy et al. (2007) showed mean concentrations of DP of 0.054 ng/g lw in whitefish, 0.430 ng/g lw in mussels, 0.450 ng/g lw in burbot, and 0.816 ng/g lw for goldeye (n=5 for each species). Recently, Houde et al (2014) reported that DP was not detected in whole body homogenates of yellow perch samples from the St Lawrence River, Quebec.
As for DP monitoring in northern regions, DP was not detected (detection limit not specified) in beluga blubber (Shen et al. 2012), and in the majority of fish muscle and liver samples analyzed in Nordic countries (TemaNord 2011). DP was measured in fish liver from Norway (Schlabach et al, 2011), blue mussel from Iceland (Schlabach et al, 2011), perch from Finland (TemaNord 2011), and halibut from Greenland (Kakimoto et al. 2012); however, concentrations were generally below the upper bound value (7.2 ng/g lw) measured in Lake Ontario lake trout (Ismail et al. 2009). DP was not detected in caribou in a food web study in the Canadian Arctic (personal communication, Aquatic Contaminants Research Division, Environment and Climate Change Canada October; November 2014; unreferenced).
Daily intake estimates of DP for the general population from the consumption of infant formula, cereal products, mixed dishes and soups food groups were based on the maximum concentrations of formula, cereal and mixed purée, respectively, measured in samples purchased in 2013 in U.S. stores (Liu et al. 2014). Daily intake estimates of DP from the consumption of fish were estimated based on the maximum concentration of 11 ng/g lw (or 1.6 ng/g ww) measured in Lake Ontario lake trout (Ismail et al. 2009). This concentration is considered appropriate for deriving upper-bounding intakes for the general population of Canada given the assumption that DP is present at this concentration in 100% of fish, shellfish, and related food items. Although certain northern populations in Canada may consume larger quantities of seafood or game in their diet, seasonally, this estimate is considered conservative enough to account for this variability.
10.1.1.7 Breast Milk
DP biomonitoring in breast milk has been reported in Canada (i.e., Siddique et al. 2012; Zhou et al. 2014) and China (Ben et al. 2013; Appendix E). DP was detected in greater than 85% of breast milk samples (n = 87) collected from two Canadian cities: Kingston, Ontario (n=39; collected in 2003–04) and Sherbrooke, Quebec (n=48; collected in 2008-09). DP concentrations in the Kingston samples ranged from not detected (detection limit = 0.05 ng/g) to 6.4 ng/g lw, and from not detected to 8.0 ng/g lw in the Sherbrooke samples. The median concentration for samples from the Kingston cohort was 0.74 ng/g lw and 95th percentile of 3.4 ng/g lw. The median and 95th percentile concentrations for the Sherbrooke cohort were 0.58 ng/g and 2.1 ng/g lw (Siddique et al. 2012).
More recently, DP was detected in 55% of breast milk samples collected in 2008-09 from a separate and larger (n=105) cohort of nursing women from Sherbrooke, Quebec (Zhou et al. 2014). In this study, DP concentrations ranged from not detected (detection limit = 0.010 ng/g) to 15 ng/g lw with a median of 0.074 ng/g lw and 95th percentile of 3.5 ng/g lw. A limitation in this study included the unrecorded timing of sampling--sampling of each subject may have occurred from delivery to six months following delivery (Zhou et al. 2014). DP was also measured in breast milk from mothers residing both near and at a distance from heavy e-waste recycling activities in China (Ben et al. 2013). DP concentrations in mothers near e-waste areas (median of 4.46 ng/g lw; n=44) were higher than those residing away from these areas (median of 2.19 ng/g lw; n=44) (Ben et al. 2013), and concentrations in both areas were higher than reported in the Canadian studies.
Daily dietary exposure estimates were obtained for nursing infants based on the 95th percentile concentration of 0.19 ng/g ww (converted to 0.06 μg/L whole milk based on a breast milk density of 1.03 g/mL) from participants from Sherbrooke, Quebec (Zhou et al. 2014; ww concentrations obtained from personal communication with EHSRB, Health Canada, dated May 15, 2014).
10.1.2 Consumer Products
DP is an additive flame retardant used to treat several polymers used in various applications such as electrical wire coatings, hard plastic computer and TV connectors, plastic decorations, and roofing material (Weil and Levchik 2004; Oxychem 2004; see section 5), and these uses can be reasonably expected in Canada. In Canada, confirmed uses of DP include automobile manufacturing, such as sensor assemblies (ECCC 2013-2014). DP in plastics range from 8% in polybutylene terephthalate (PBT) up to 40% in silicon rubber (OxyChem 2007).
DP has not been identified to be present in consumer products intended for children, such as toys, that would lead to frequent exposure for children. Also, in preliminary consumer product testing conducted by Health Canada, DP was not detected (LOQ of 0.3%) in 39 subsamples collected from 23 children's manufactured items (e.g., foam chair, nursing pillows, toys, etc.) purchased in retail stores in Ottawa, Ontario, in January and May 2014 (Health Canada 2014). While children have been observed to opportunistically mouth a wide variety of objects not intended for mouthing, including electrical and electronic products, the frequency and duration of mouthing of these objects is not expected to be higher than those from mouthing of toys or other manufactured items intended for children (Juberg et al. 2001).
As an additive flame retardant, DP may be released from treated polymers through abrasion; however releases due to leaching or migration are expected to be limited by the physical-chemical properties of this substance. Due to DP's low vapour pressure, inhalation exposure is expected to be low. The negligible water solubility of DP also limits the potential for water, sweat or saliva-mediated transfer or migration. Based on current information regarding DP's use profile and physical-chemical properties, potential for exposure of the Canadian general population to DP from manufactured items, including vehicles, is low, and exposure estimates were therefore not derived. Also, exposure from manufactured items is expected to be accounted for indirectly through estimates of exposure to DP in indoor air and dust (see sections 10.1.1.2 and 10.1.1.3 ).
In addition to human milk (section 10.1.1.7 ), DP has been measured in several biological matrices including blood serum, placental tissue, and cord serum (Appendix F).
In Canada, DP isomers were detected in the majority (87%; LOD 0.12 ng/g lw) of maternal blood serum samples (n=102) collected in 2008-09 from mothers following delivery in Sherbrooke, Quebec (Zhou et al. 2014). DP concentrations in serum ranged from not detected (detection limit = 0.08 ng/g lw) to 81 ng/g lw, with a median of 2.4 ng/g lw and a 95th percentile of 31.6 ng/g lw. The ratio of the two DP isomers found in human serum samples, calculated as anti/(anti + syn), was found to be 0.8 which is similar to the ratio reported in the DP technical mixture.
In Europe, DP was also frequently detected (94%; LOQ of 0.16 ng/g lw) in a 48 banked serum samples collected in France (Brasseur et al. 2014), as well in serum samples collected from 46 women in Norway participating in a mother-child cohort study (no samples were collected from children) (Cequier et al.2015). Serum samples collected in France in 2003-05 were from an equal number of women and men residing within the vicinity of a municipal solid waste incinerator, where DP concentrations ranged from not detected to 7.04 ng/g lw with a median concentration of 1.2 ng/g lw. In samples collected from Norwegian women, DP concentrations ranged from not detected to 31 ng/g lw with a median concentration of 1.3 ng/g lw. In a separate mother-toddler paired study in Sweden involving 24 mtohers and their toddlers (11-15 months), DP was not frequently detected (mLOQ of 140 pg/sample for the anti isomer). DP was detected in serum from one toddler (148 ng/g lw; individual isomeric values summed) and one mother (88 ng/g lw; individual isomeric values summed). These samples were not from the same household (Sahlstrom et al. 2014). No production source has been identified in Europe (section 4).
Several studies in China have monitored DP in biological matrices to determine the extent of exposure from electronic-waste recycling facilities (e.g., Ren et al. 2009; Yan et al. 2012; He et al. 2013; Yang et al. 2013; Ben et al. 2014). Median concentrations of DP in serum collected from low-exposure groups, e.g., participants residing away from e-waste activity, were measured up to 13.7 ng/g lw (Ren 2009; Yang et al. 2013), and were statistically lower than their respective paired groups associated to higher e-waste exposure. DP median concentration in serum were measured up to 265 ng/g lw in samples collected in 2011 from male (n=33) and female (n=37) participants from e-waste recycling sites (Yan et al. 2012).
Ben et al. (2014) investigated transplacental transfer of DP from 72 residents of the e-waste recycling area of Wenling, China. Samples of maternal serum, placenta and cord serum were collected in 2010-11, and DP concentrations in all three matrices were strongly correlated. Median DP concentrations of maternal sera, placental tissue and cord blood sera for the high-exposure group (n=48; lived in Wenling for more than 20 years) were found to be 8.43, 3.21 and 2.82 ng/g lw, and were statistically higher than those for the low-exposure group (n=24; lived in Wenling for less than 3 years), at 3.55, 1.09 and 1.82 ng/g lw, respectively. The authors highlight that the presence of DP in cord sera is indicative of potential DP translocation from maternal to fetal tissues. DP dechlorinated products, or potential metabolites, in humans have also been detected in some Chinese studies (e.g., Ren et al. 2009 and Ben et al. 2014); however, the sources of these dechlorinated products (i.e., whether produced in vivo or from the external exposure) were not determined.
DP measured in serum may provide a measure of integrated exposure, from various routes (oral, dermal, and inhalation) and all sources of exposure, including environmental media, diet and products. The DP human serum levels (2.4 ng/g lw; 50thpercentile) were lower than levels in the rat control group (expected to be exposure via food and dust; 61 ng/g lw) and the group exposed to the highest does of 100 mg/kg-bw/d for 90-day (690 ng/g lw) as reported in the rat toxicokinetics study (Li et al. 2013) described further in section 10.2.7. While directly comparing human and rat serum data may provide an opportunity to evaluate internal exposures in humans (Aylward and Hays 2011), given the unusual distribution patterns observed for DP in rats in the Li et al. study, and the limited information on toxicokinetics in both rats and humans, the comparison between human and rat serum is limited.
10.2 Health Effects Assessment
No classifications of the health effects of DP by national or international regulatory agencies were identified. The US EPA has developed screening-level hazard characterization documents for DP (US EPA 2011, 2014).
No chronic toxicity or carcinogenicity studies were identified. Several other lines of evidence were investigated to assess the carcinogenic potential of DP (more detail is available in Health Canada 2015). The OECD (Q)SAR Toolbox (OECD 2009b, 2011, 2012) and OASIS TIMES (TIMES 2012) were used to identify potential analogues. Overall, no appropriate analogue could be identified. The quantitative structural activity relationship (QSAR) approach used several statistically based (Q)SAR models to assess the carcinogenic potential of DP. The (Q)SAR models generated mixed results with low confidence in the overall prediction. The third approach was to identify any structural alerts associated with carcinogenicity using computer models. One of the structural alert screening models out of 2 sets of models triggered an alert for non-genotoxic carcinogenicity based on the presence of the polyhalogenated cycloalkane fragment. Overall, results remain inconclusive.
In terms of in vitro genotoxicity, results from Ames assays conducted using Salmonella typhimurium strains (TA98, TA100, TA1535, TA 1537 and TA 1538) were negative in the presence or absence of metabolic activation (S9) (Mortelmans and Tanaka 1980). Result from an in vitro mouse lymphoma assay was also negative in the presence and absence of S9 (Jotz and Mitchel 1980).
One in vivo genotoxicity study was identified. Mice were orally administered 0, 500, 2000 or 5000 mg/kg-bw/day of DP via gavage for 10 days (Wu et al. 2012). Liver samples were collected for a comet assay and the genotoxicity result was negative.
10.2.3 Acute Toxicity
Acute toxicity studies in experimental animals suggested low concern for acute toxicity via the oral, inhalation or dermal routes of exposure (Powers 1964; Moldovan 1971a, b; Kinert 1975).
10.2.4 Repeated-dose Toxicity
No adverse health effects were observed in any of the identified repeated-dose oral toxicity studies, testing dose levels up to 5000 mg/kg-bw/day.
In a recent study conducted by Wu et al. (2012), male ICR mice (6/dose) were exposed to DP in corn oil by oral gavage at 500, 2000 or 5000 mg/kg-bw per day for 10 days. Body weights and organs weights (liver, kidney, and testes) were not affected by DP treatment. The study examined hepatic oxidative stress, DNA damage and transcriptomic and metabonomic profiles at the molecular level. Some oxidative stress responses and alteration of gene expression involved in carbohydrate, lipid, nucleotide, and energy metabolism and signal transduction processes were observed. As no health effects were observed, these physiological changes were not considered adverse effect.
In a combined repeated-dose/reproductive/developmental toxicity screening test, conducted according to OECD guidelines, Crl:CD (SD) rats were exposed to DP in corn oil at 0, 750, 1500 or 5000 mg/kg-bw/day by oral gavage (Brock et al. 2010). In the repeated dose toxicity (RDT) phase, animals (10/sex/dose) were treated for 28 days. No treatment-related effects were observed on clinical signs of toxicity, body weights, food consumption, neurobehavioral, and functional observational battery evaluations. No effects were observed on haematology, urinalysis, coagulation or clinical chemistry parameters. No dose response-related changes in organ weights (heart, liver, testes, ovaries, and thyroid/parathyroid glands) were observed. Mortalities were observed across all dose groups including controls, which were linked to gavage administration errors (Table 10-1). The authors identified a no-observable-effect level (NOEL) of 5000 mg/kg-bw/day.
|Dose (mg/kg-bw/day)||Mortality in male rats||Mortality in female rats|
Li et al. (2013) examined potential health effects on rats at lower dose levels. Sprague-Dawley male rats (7/dose) were administered DP via oral gavage in corn oil at 0, 1, 10 or 100 mg/kg-bw/day for 90 days. There were neither significant changes in body weight nor changes in absolute and relative liver weights. No histopathological liver damage was observed. Other organs were not examined. In terms of clinical chemistry parameters, significant decrease activities of alanine amino transferase (ALT), alkaline phosphatase (ALP) and total bile acids (TBA), and increase in levels of glucose were observed at 100 mg/kg-bw/day.
In another subchronic study, Charles River strain albino rats (15/sex/dose) were administered DP in their diets at 0, 10 000, 30 000 and 100 000 ppm (0, 500, 1500 and 5000 mg/kg-bw per day, respectively) for 13 weeks (Oscarson 1975). There were no statistically significant treatment-related effects on body or organ (brain, gonads, heart, kidney, and spleen) weights, urinalysis, clinical chemistry or haematology. There were no treatment-related clinical signs or gross pathological or histopathological findings. Absolute and relative (to body and brain weight) liver weights were increased in high-dose animals but the absolute liver weight was not statistically significant in males and these increases were not associated with any histopathological lesions.
One repeated-dose inhalation toxicity study was identified. COBS rats (5/sex/dose) were exposed to DP at 0 (untreated control), 640 or 1524 mg/m3, 6 hours per day, 5 days per week for 28 days (Bishop 1975). Animals were exposed in exposure chamber with DP in the form of dust. Starting from 640 mg/m3, a significant increase in absolute liver weight was observed in both sexes. Corresponding hepatocytomegaly (swelling of liver cells with signs of cytotoxicity and necrosis) of centrilobular hepatocytes was observed in males starting from 640 mg/m3 and in some females at the next concentration. Effects on lungs were observed. All treated animals exhibited a slight increase in the number of macrophages in the alveoli. A significant increase in absolute lung weight was observed in females starting from 640 mg/m3 and at 1524 mg/m3 in males. A lowest-observed-adverse-effect concentration (LOAEC) of 640 mg/m3 was identified in this study. No NOAEC was identified in this study.
One repeated-dose dermal toxicity study was identified. Male and female New Zealand White rabbits (5/sex/group) were administered DP in 3% aqueous methylcellulose at 0 (untreated control), 500 or 2000 mg/kg-bw per day, 5 days per week for 4 weeks (Trzyna 1975). The test substance was distributed over 20% of the total body surface area on shaved abraded skin. The application sites were not occluded, but animals were individually housed and fitted with Elizabethan collars throughout the study. The only treatment-related clinical sign was none to minimal erythema at the application site. Females exhibited statistically significant, dose-related decrease in absolute and relative (to body and brain weight) gonad (combined uterus and ovaries) weights starting from 500 mg/kg-bw/day and a significant decrease in absolute and relative (to body and brain weight) liver weight at 2000 mg/kg-bw/day. As no corresponding histopathology was observed in these organs (liver and ovary), a NOAEL of 2000 mg/kg-bw/day was identified for this study, in the screening-level hazard documents by US EPA (US EPA 2011, 2014a).
10.2.5 Reproductive and Developmental Toxicity
Potential reproductive and developmental effects of DP were explored in a combined repeated-dose/reproductive/developmental toxicity screening test described above (Brock et al. 2010). In the developmental and reproductive toxicity (DART) phase, animals (20/sex/group) were administered 0, 750, 1500 or 5000 mg/kg-bw/day DP in corn oil via oral gavage. The male rats were treated for at least 63 days (21 days premating, 14 days of mating, 28 days after mating). The female rats were treated for up to 60 days (21 days during premating, 14 days of mating and up to 25 days after mating from gestation day (GD) 0 to lactation day (LD) 3). Mortalities were observed across all dose groups including controls, which were linked to gavage administration errors (Table 10-2). No effects on reproductive or fertility indices were observed in either male or female parental animals. No maternal toxicity was observed, up to the highest dose level tested. No developmental effects were observed in the F1 offspring, up to the highest dose level tested. The authors identified NOELs of 5000 mg/kg-bw/day for reproductive and developmental toxicity.
|Dose (mg/kg-bw/day)||Mortality in male rats||Mortality in female rats|
No skin sensitization was observed in guinea pigs treated with DP in a Buehler's test (Brett 1975).
Based on two toxicokinetic studies (Chou et al. 1979; Saunders and Quistad 1983; OxyChem 2005) where rats were orally administered radiolabeled DP, it was found that DP was poorly absorbed from the gastrointestinal tract. In the Chou et al. (1979) study, at least 75% of radiolabelled DP (suspended in water containing 5% each of Tween-80 and gum arabic) was excreted in faeces 24 hours post-treatment. In the Saunder and Quistad (1983) study, at least 83% of radiolabelled DP (vehicle not specified in secondary source) was excreted in faeces 4 days following treatment (OxyChem 2005). Less than 0.1% of DP was excreted in urine in both studies. Radioactivity was detected in tissue samples with the highest levels found in liver and ovaries. Lower levels were detected in blood, kidney and lung. DP was excreted very slowly where the levels of DP in blood, kidney and liver remained unchanged between 4 and 24 hours post-treatment (Chou et al. 1979). A polar metabolite(s) was detected but was not identified in either study.
The Li et al. (2013) study also examined the accumulation pattern of the two isomers of DP (syn-DP and anti-DP) in rats. Distribution patterns showed that both isomers of DP were measured, with the highest level in liver followed by serum and then muscle. Dechlorinated DP isomers (Cl11-DP) were measured in these tissues. Since Cl11-DP was also detected in commercial DP, it was not clear if Cl11-DP was a metabolite or originated from the commercial product. The commercial DP contains the two isomers (syn-DP and anti-DP) with the ratio of 1:3. At the low dose level of 1 mg/kg-bw/day, no stereoselectivity of anti-DP or syn-DP in tissues (muscle, liver and serum) was observed based on the ratio of anti-DP concentration to total DP concentration (fanti) that was comparable to the composition of commercial DP. At higher dose levels (10 and 100 mg/kg-bw/day), the fanti was lower, suggesting higher levels of syn-DP in tissues. Two separate groups of animals were exposed to 0 or 100 mg/kg-bw/day of DP for 45 days followed by 45 days depuration to examine elimination patterns. It was found that both isomers of DP were more prone to accumulate in liver or eliminated slower in liver than in muscle. The elimination half-lives were 24 days (syn-DP) and 25 days (anti-DP) in serum, 44 days (syn-DP) and 54 days (anti-DP) in muscle, and 179 days (syn-DP) in liver. The authors did not calculate the elimination half-life for anti-DP in liver since level of the anti-DP increased non-significantly after depuration.
10.3 Characterization of Risk to Human Health
No classifications of the health effects of DP by national or international regulatory agencies were identified. Results for the genotoxicity database were all negative, indicating that DP is not likely to be genotoxic. No chronic studies were identified. Analyses from other lines of evidence to assess the carcinogenic potential of DP were inconclusive.
There were no adverse effects observed in experimental animals orally treated with DP with dose level up to 5000 mg/kg-bw/day in a combined 28-day repeated-dose toxicity study with a reproductive/developmental toxicity screening test (Brock et al. 2010) and in a 90-day subchronic toxicity study (Oscarson 1975), both conducted in rats. There are eight orders of magnitude between this no effect level and the upper-bounding estimate of exposure to DP for the Canadian general population from environmental media (air, water, dust) and food and breast milk (8.2 ng/kg-bw/day for young children 0.5-4 years of age). This margin is considered to be adequate to account for uncertainties in the exposure and health effect databases.
10.4 Uncertainties in Evaluation of Risk to Human Health
This draft screening assessment acknowledges uncertainties regarding the exposure database and health effects database.
No data in the primary literature were available for marketed foods in Canada; however data were available for baby food products in the U.S.
No volunteer or epidemiological studies were identified. In experimental animals, no chronic studies were available as the longest tested studies were only up to 90 days and sample size for the repeated-dose toxicity studies were relatively small. There are uncertainties whether pregnant animals might exhibit different toxicokinetics and whether there are differences in sensitivity in chemical-induced effects in developing pups than adult animals.
Given the large magnitude of the margin of exposure, the uncertainties in the health effect database is considered to be accounted for.
While assessment of "dechlorane-related" substances (described in section 2.2) is beyond the scope of this screening assessment, and although it is not well understood how similar these substances are to DP, there is an uncertainty with respect to the potential for co-exposure as well as common mode of action of "dechlorane-related" substances.
Considering the information presented in this draft screening assessment, it is proposed to conclude that DP is entering the environment in a quantity or concentration or under conditions that have or may have an immediate or long-term harmful effect on the environment or its biological diversity, or that constitute or may constitute a danger to the environment on which life depends. Additionally, DP meets both the persistence and bioaccumulation criteria as set out in the Persistence and Bioaccumulation Regulations (Canada 2000).
Based on the available information on its potential to cause harm to human health, it is proposed to conclude that DP is not entering the environment in a quantity or concentration or under conditions that constitute or may constitute a danger in Canada to human life or health.
11.1 Overall Conclusion
It is proposed to conclude that DP meets one or more of the criteria set out in section 64 of CEPA. DP is proposed to meet the persistence and bioaccumulation criteria as set out in the Persistence and Bioaccumulation Regulations of CEPA.
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Appendix A: Structural Identity
|CAS RN||Other selected namesa|
IUPAC name: (1,6,7,8,9,14,15,16,17,17,18,18-Dodecachloropentacyclo[220.127.116.11,9.02,13.05,10]octadeca-7,15-diene);
Dechlorane Plus 1000;
Dechlorane Plus 25;
Dechlorane Plus 2520;
Dechlorane Plus 35;
Dechlorane Plus 515;
a Names acquired from: US EPA 2014 Alternatives assessment for Flame Retardant DecaBDE, Bergman et al. 2012, OxyChem MSDS etc.
A-2. Selection of analogues and use of (Q)SAR models
The analogues used to inform the sediment and soil toxicity sections of this ecological assessment are presented in Table 2-2. DP is a replacement for the flame retardant use of organochlorine Mirex (also called Dechlorane, CAS RN 2385-85-5), and therefore Mirex was identified as a potential analogue. Chlordane was also identified by the OECD QSAR Application Toolbox as a structurally and functionally close analogue for which sediment and soil toxicity data were available. DP, Chlordane, and Mirex (as well as other "dechloranes") are all similarly synthesized from hexachlorocyclopentadiene and are expected to behave similarly in the environment (e.g., partitioning to soil and sediment, stable/persistent etc.) (Environment and Climate Change Canada 1977).
While indices for the analogues vary in predicted similarity (DP and Chlordane are 59.14% (Tanimoto Index) to 92.6694% (Chem ID structural similarity), Mirex and DP are 67.35% (Chem ID structural similarity) to 68.94% (Tanimoto Index), the structures (e.g., molecular weight, degree of chlorination) and reactivity profiles support that the substances are appropriate analogues for soil and/or sediment ecotoxicity. Using Chlordane and Mirex as analogues for toxicity is likely conservative as they are more bioavailable and therefore likely more toxic than DP (at least to aquatic organisms), due to their higher water solubility. As a result, these analogues were considered appropriate for use in the ecological effects assessment section (for sediment and soil organisms) to represent DP.
Although Mirex was never registered for use as a pesticide in Canada, it has been used worldwide as a stomach insecticide (formulated in baits) for ant and other insect pests and as a flame retardant in plastics, rubber, paint, paper and electrical goods (Environment and Climate Change Canada 2014, IPCS 1984). Mirex appears on the List of Toxic Substances (Schedule 1) of the Canadian Environmental Protection Act, 1999 (CEPA), and is regulated under the Mirex Regulations, 1989.
Chlordane is an organochlorine pesticide that was used in Canada from the mid-1940s to the 1980s, but its registration and use under the Pest Control Products Act were discontinued as of 1991 (CCME 1999). Chlordane appears on the List of Toxic Substances (Schedule 1) of CEPA.
Chlordane occurs in several isomeric forms, the most common of which are the (cis) and (trans) forms (USEPA 1979). The IUPAC chemical name for the cis isomer of Chlordane is 1,2,4,5,6,7,8,8-octachloro-3a,4,7,7a-tetrahydro-4,7-methanoindane and for the trans isomer is 1,2,4,5,6,7,8,8-octachloro-3a,4,7,7a-hexahydro-4,7-methano-1H-indene. Technical Chlordane is a mixture of more than 140 chlorinated hydrocarbons (ATSDR 1989a); technical grade Chlordane is approximately 24% g-isomer, 19% a-isomer, 10% Heptachlor, 21% chlordene isomers, 7% nonachlor, and 18.5% related chlorinated compounds (Environment and Climate Change Canada 1998).
Appendix B: Physical-Chemical Properties
Physical-chemical properties of DP were checked for internal consistency according to the Least-Squares Adjustment Procedure (LSA) (Schenker et al. 2005). To conduct this, the geometric mean or arithmetic mean (for log partition coefficients) of available values for each physical-chemical parameter (vapour pressure, water solubility, octanol solubility, log Kow, log Koa, log Kaw) was entered into the model. Sub-cooled values were used for vapour pressure, water solubility, and octanol solubility. The values used to determine these means represent the most reliable and independent values available from empirical and modelling data (Table B-1; for all Physical-Chemical values see Table B-2). In determining internal consistency of the properties, the LSA model also produces predicted values (Table B-1).
While experimental based estimates for water solubility and vapour pressure exist for DP, there remains uncertainty with these values. For the purposes of this assessment, the log Kowvalue 8.78, derived from the LSA method, was selected. To maintain internal consistency of physical chemical values, the LSA method value for water solubility and vapour pressure were also considered. Final selected values are summarized in Table 2-1. Generally, DP is characterized by very low water solubility, low to very low vapour pressure, and a very high organic carbon-water partition coefficient and octanol-water partition coefficient.
|Data Source||Vapour Pressure (Pa)||Water Solubility|
|Log Kow||Log Kaw||Log Koa|
|Experimental DP||-||6.12 x 10-8||4.67 x 10-7d||-||-||-|
|Experimental DP||-||3.81 x 10-7||0.719||-||-||-|
|1.53 x 10-9||6.77 x 10-13a||-||11.27||-3.52||14.79|
|Episuite Model||1.01 x 10-8||1.0 x 10-9b||-||-||-||-|
|Episuite Model||3.57 x 10-11||-||-||-||-||-|
|VCC/AGLOGs||-||1.28 x 10-6||-||8.29||-||-|
(subcooled liquid value in brackets)
|8.20 x 10-10|
(1.35 x 10-6)
|1.58 x 10-8|
(2.60 x 10-5)
|5.79 x 10-4|
(9.52 x 10-1)
(subcooled liquid value in brackets)
|1.08 x 10-7|
(6.57 x 10-11)
|7.18 x 10-7|
(4.36 x 10-10)
(2.62 x 10-1)
Geomean/mean Input Values"-" : No data.
a WSKOWWIN 2010.
b WATERNT 2010 (fragment method).
c Calculated arithmetic mean for log values since equivalent to geometric mean for antilog values (of partition coefficients).
d In order to maximize independence of parameter estimates, model values reliant on user logkow value were not included in the calculation of water solubility geomean.
e All data sources in Table B-2 (note unit conversions for water solubility), other than octanol solubility (from US EPA (2009) and ECHA (2013a). Individual listed values are solid state values for WS, VP, and Octanol solubility; the subcooled liquid state LSA Geomean/mean iInput and Output Values are in brackets beneath.
|Physical state||Experimental||White, Crystalline Powder||NA||Occidental Chemical Company 2004|
|Melting point (°C)||Experimental||greater than 325||NA||Merck Index 2001|
|Melting point (°C)||Experimental||350||NA||USEPA 2009|
|Melting point (°C)||Modelled||349.84||N/A||MPBPWIN 2010|
|Melting point (°C)||Modelled||170.60||N/A|| MPBPWIN 2010|
(Adapted Joback Method)
|Melting point (°C)||Modelled||260.22|
(Gold and Ogle Method)
|Boiling point (°C)||Modelled||486.83|
Irrelevant; expected to degrade before boiling
38-42 lb/ ft3 (0.61-0.67 g/cm3
(DP-515 and DP-25)
|NS||US EPA 2009|
|Density (kg/m3)||Experimental||25-30 lb/ft3 (0.4-0.48 g/cm3)|
|NS||US EPA 2009|
(6 x 10-3 mmHg)
|200||US EPA 2009|
|Modelled||1.53 x 10-9|
(1.15 x 10-011 mm Hg)
(Modified Grain method)
|Modelled||1.01 x 10-8|
(7.59E-011 mm Hg)
|Modelled||3.57 x 10-11|
(2.68 x 10-13mmHg)
|Modelled||1.08 x 10-7|
|25||Least-Squares Adjustment Method (LSA) output Schenker et al. 2005|
|Modelled||6.57 x 10-11|
|25||(LSA) output Schenker et al. 2005|
|Henry's Law constant|
(7.44 x 10-6 atm·m3/mole, 3.04 x 10-4 unitless)
|25||HENRYWIN 2011 (Bond method)|
|Henry's Law constant|
(log Kaw= -3.28))
|25||LSA Schenker et al. 2005|
|Modelled||8.78b||25||LSA Output Schenker et al. 2005|
|6.653||NS||Chou et al. 1979|
(estimate from MCI) 7.76d
|Modelled||12.99b||25||LSA Schenker et al. 2005|
(249ppb; average of 197 ppb, 301ppb)
|25||Scharf DJ. 1978|
|Experimental||~4.0 x 10-5|
(207 ng/L and 572 ng/L for individual isomers)e
|22||Chou et al. 1979g|
|Experimental||less than 1.67 x 10-6f||20||ECHA 2013b|
|Modelled||4.42 x 10-10c||25||WSKOWWIN 2010|
|Modelled||7.64 x 10-6d||25||WSKOWWIN 2010|
|Modelled||6.54 x 10-7||25||WATERNT|
|Modelled||8.4 x 10-4||25||ALOGPS 2.1|
|Modelled||5.0 x 10-4||25||ACD/Percepta|
|Modelled||4.69 x 10-4 (subcooled liquid)b||25||LSA Output Schenker et al. 2005|
|Modelled||2.85 x 10-7|
(solid; i.e., non-subcooled)
|25||LSA Output Schenker et al. 2005|
Abbreviations: log Kow = octanol-water partition coefficient; log Koc = organic carbon-water partition coefficient; log Koa = octanol-air partition coefficient; pKa = acid dissociation constant; N/A = not applicable.
a Values in parentheses represent the original ones as reported by the authors or as estimated by the models.
b For Least Squares Adjustment Method (LSA), see Table B-1 for list of values used.
c Used log Kow = 11.27 (KOWWIN).
d Used log Kow= 8.78 (Least Squares Adjustment Method -LSA).
e USEPA (2009) HPV (IUCLID Data Set of 07-Nov-2008) indicated that indirect result from the sediment-water partitioning experiment suggested that the solubility was about 44 ng/L (total for both isomers). This lower value was also considered the best estimate of water solubility.
f ECHA (2013b) water solubility report summary was published after the data analysis and modelling was completed for the draft DP assessment, and therefore was not included in the Least Squares Adjustment Method (LSA) for determining selected physical-chemical properties. However, the ECHA (2013b) reported result is very similar to the water solubility value selected in this assessment, and will be considered for the final assessment.
|Property||Vapour pressure (Pa)||Log Kow||Log Koc||Water solubility|
|chlordane||1.0 x 10-5||5.2 - 6.0||4.78||0.56|
|mirex||1. x 10-4||6.89||6||0.085|
aPhysical-chemical properties as cited in Environment and Climate Change Canada (1998).
bExperimental physical-chemical properties as presented in Episuite (2000-2012).
Appendix C: Upper-bouding estimates of daily intake (μμg/kg bw/d) of DP by various age groups within the general population of Canada
|Route of Exposure||0-6 moa|
Not Formula fedd
|0.5-4 yre||5-11 yrf||12-19 yrg||20-59 yrh||greater than or equal to 60 yri|
Abbreviations: N/A = not applicable; mo = months; yr = years.
a Assumed to weigh 7.5 kg, to breathe 2.1 m3 of air per day (Health Canada 1998), and to ingest 38 and 0 mg of dust and soil per day, respectively (Wilson et al. 2013).
b Exclusively for breast fed infants, assumed to consume 0.742 L of breast milk per day (Health Canada 1998), and breast milk is assumed to be the only dietary source. The 95th percentile concentrations of total DP (0.054 ng/g wet weight) in breast milk samples (n=102) collected in 2008-09 from women from Sherbrooke, Quebec, Canada (Zhou et al. 2014; personal communication from EHSRB, Health Canada, dated May 15, 2014), multiplied by a breast milk density of 1.03 g/mL (converted to 0.06 ug/L), was selected for deriving upper-bounding daily intakes of DP for breast milk exposure.
cExclusively for formula-fed infants, assumed to drink 0.8 L of water per day (Health Canada 1998), where water is used to reconstitute formula. See footnote on food and footnote on water for details.
d Exclusively for not formula-fed infants, assumed to drink 0.7 L of water per day, and to consume 45.1 g of cereal products per day and 99.1 g of mixed dishes and soups per day (Health Canada 1998). Approximately 50% of non-formula-fed infants are introduced to solid foods by 4 months of age, and 90% by 6 months of age (NHW 1990).
e Assumed to weigh 15.5 kg, to breathe 9.3 m3 of air per day, to drink 0.7 L of water per day, to consume 54.7 g of fish per day, 162.2 g of cereal products per day, and 149.1 g of mixed dishes and soups per day (Health Canada 1998), and to ingest 41 and 14 mg of dust and soil per day, respectively (Wilson et al. 2013).
f Assumed to weigh 31.0 kg, to breathe 14.5 m3 of air per day, to drink 1.1 L of water per day, to consume 89.8 g of fish per day, 290.1 g of cereal products per day, and 180.0 g of mixed dishes and soups per day (Health Canada 1998), and to ingest 31 and 21 mg of dust and soil per day, respectively (Wilson et al. 2013).
g Assumed to weigh 59.4 kg, to breathe 15.8 m3 of air per day, to drink 1.2 L of water per day, to consume 97.3 g of fish per day, 320.9 g of cereal products per day, and 213.0 g of mixed dishes and soups per day (Health Canada 1998), and to ingest 2.2 and 1.4 mg of dust and soil per day, respectively (Wilson et al. 2013).
h Assumed to weigh 70.9 kg, to breathe 16.2 m3 of air per day, to drink 1.5 L of water per day, to consume 111.7 g of fish per day, 248.4 g of cereal products per day, and 220.5 g of mixed dishes and soups per day (Health Canada 1998), and to ingest 2.5 and 1.6 mg of dust and soil per day, respectively (Wilson et al. 2013).
i Assumed to weigh 72.0 kg, to breathe 14.3 m3 of air per day, to drink 1.6 L of water per day, to consume 72.9 g of fish per day, 229.0 g of cereal products per day, and 213.8 g of mixed dishes and soups per day (Health Canada 1998), and to ingest 2.5 and 1.5 mg of dust and soil per day, respectively (Wilson et al. 2013).
j The maximum concentration of 340 pg/m3, measured in the Great Lakes region (Burdt Island, Ontario; Hung et al. 2014), was selected for deriving upper-bounding estimates of daily intake for air exposure.
k No drinking water monitoring data were identified. The DP mean concentration of 13.9 pg/L from Lake Ontario, the highest among the Great Lakes (Venier et al. 2014), was selected for deriving upper-bounding estimates of daily intake for drinking water exposure.
l No monitoring data on marketed foods in Canada were identified; however data on three baby food categories were identified for samples collected in the U.S. (Liu et al. 2014). DP maximum concentrations in formula (83.2 pg/g ww), cereal (427 pg/g ww) and purée (23.6 pg/g ww) from the U.S. were selected for deriving upper-bounding estimates of daily intake for exposure to infant formula (infant formula fed group only), cereal products (infant not formula-fed and all higher age groups), and mixed dishes and soups food groups (infant not formula-fed and all higher age groups), respectively. The upper bound mean concentrationin of 0.85 ng/g ww (7.2 ng/g lw) in Lake Ontario lake trout (Ismail et al. 2009) was selected for deriving upper-bounding estimates of daily intake for exposure to all fish-related food items in the fish food group. Amounts of foods consumed on a daily basis by each age group over 12 food groups were obtained from the 1970-1972 Nutrition Canada Survey (Health Canada 1998).
m The total DP 95th percentile concentration (107.4 ng/g) from the Canadian baseline study (n=413) was selected for deriving upper-bounding estimates of daily intake for dust exposure.
n No monitoring data of soil in North America were identified. Therefore, the maximum soil predicted environmental concentration (PEC) of 59 000 ng/g dw (0.059 mg/kg dw) was selected for deriving upper-bounding estimates of daily intake for soil exposure.
Appendix D: Concentrations of DP in household dust
|Location||Sample Type||Sampling Year||Sample Size||Median|
|Various, Canada||Vacuum||2007- 2008||413||11.1|
[less than mdl (1.2 ng/g for syn-DP; 1.9 ng/g for anti-DP) to 2982]
|107.4||Kubwabo et al. 2014 (unpublished)|
|Ottawa, ON, Canada||Vacuum||2002- 2003||69||14|
[2.3 - 182]a
|121||Zhu et al. 2007|
|Ottawa, ON, Canada||Vacuum||2007||7||22|
[14 - 61]
|60||Zhu et al. 2007|
|Vancouver, BC, Canada||Vacuum||2007-2008||116||6.8|
[less than 0.8-354]
|57.7||Shoeib et al. 2012|
|Toronto, ON, Canada (TI)||Vacuum||2010-2011||5||8.2 (mean)|
[DL to 35]
|NS||Diamond et al. 2013|
|Toronto, ON, Canada||Vacuum||2012||20||30.9 (mean)|
[less than 0.010 - 170]
|NS||Diamond et al. 2013|
|California, USA||Living Area Surfaces||2006||16||10|
[3 - 47]
|NS||Dodson et al. 2012|
|California, USA||Living Area Surfaces||2011||16||4.5|
[less than 2 -15]
|NS||Dodson et al. 2012|
|Boston, USA||Vacuum||2002- 2003||38||13.1|
[NS - 111.5]
|44.4 (P90)||Johnson et al. 2013|
|Living Area Surfaces||2008- 2009||27||13.8|
[2.78 - 70.4]
|62.2||Wang et al. 2011|
|Rural Area, China||Living Area Surfaces||2008- 2009||20||3.95|
[ND - 27.1]
|26.8||Wang et al. 2011|
|Yuangtan Town, China|
|Living Area Surfaces||NS||10||64.9 (mean)|
[32.6 - 118]
|NS||Zheng et al. 2010|
|Yuangtan Town, China|
|Urban Living Area Surfaces||NS||27||18.9 (mean)|
[2.78 - 70.4]
|NS||Zheng et al. 2010|
Abbreviations: P95 = 95th percentile; P90 = 90th percentile; ON = Ontario; BC = British Columbia; NS = not specified; ND = not detected; TI = Toronto Intensive pilot study.
a Excluding one extreme value of 5683 ng/g.
Appendix E: Concentrations of DP in human breast milk
|Sherbrooke, QC, Canada||2008- 2009||105||less than 0.01 - 15||0.074||3.5||Zhou et al. 2014|
|Kingston, ON, Canada||2003- 2004||39||less than 0.05 - 6.4||0.74||3.4||Siddique et al. 2012|
|Sherbrooke, QC, Canada||2008- 2009||48||less than 0.05 - 8||0.58||2.1||Siddique et al. 2012|
|Wenling, China; High-exposure group||2010- 2011||44||1.01 - 590||4.46||NS||Ben et al. 2013|
|Wenling, China; Low-exposure group||2010- 2011||44||0.83 - 8.05||2.19||NS||Ben et al. 2013|
Abbreviations: lw = lipid weight; P95 = 95thpercentile; QC = Quebec; ON = Ontario; DL = detection limit; n = sample size; NS = not specified.
Appendix F: Concentrations of DP in human serum
|Location||Sampling Year; n||Range (ng/g lw)||Median (ng/g lw)||P95|
|Sherbrooke, QC, Canada||2008-2009; 102||ND - 81||2.39||31.6||Zhou et al. 2014|
|Besançon, France||2003-2005; 48||ND - 7.04||1.20||NS||Brasseur et al. 2014|
|Oslo area, Norway||46||ND- 31||1.3||NS||Cequier et al. 2014|
|Wenling, China; High-exposure group||2010-2-11; 48||Maternal:1.28 - 900|
Placentas: 0.92 - 197
|NS||Ben et al. 2014|
|Wenling, China; Low-exposure group||2010-2011||Maternal: 1.69-11.6|
|NS||Ben et al. 2014|
|Guiyu town, China (workers in e-waste dismantling industry)||2005||7.8 - 465||42.6||NS||Ren 2009|
|Haojiang, China (workers in fishing industry)||2005||0.93 - 50.5||13.7||NS||Ren 2009|
|Tianjin, China (exposure group of workers and local residents)||2009 and 2010; 35||4.21 - 12.4||6.29||Yang et al. 2013|
|Tianjin, China, (control group, in proximity to the e-waste plants but not engaged in dismantling)||2009 and 2010; 21||0.53 - 1.79||1.06||NS||Yang et al. 2013|
Abbreviations: lw = lipid weight; P95 = 95thpercentile; n = sample size; ND = not detected; NS = not specified.
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